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TranscriptomicsTranscription profiling by array of hMADS cells transfected with miR-26a and induced to differentiate into adipocytes
Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA). Two days later (=d0), adipocyte differentiation was induced. At d9 after start of differentiation, cells were harvested to identify mRNAs that are down- and upr...
ORGANISM(S): Homo sapiens 
2013-11-25 | E-MARS-29 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of hMADS cells transfected with miR-26a
Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA) and cells were harvested two days later (=d0) to identify mRNAs that are downregulated by miR-26a versus miR-C, as such mRNAs could be direct miR-26a targets.
ORGANISM(S): Homo sapiens 
2013-11-25 | E-MARS-28 | biostudies-arrayexpress
TranscriptomicsmicroRNA expression profiling of human colorectal cancer cell lines: HRT-18 vs. Caco-2
MicroRNAs regulate the biological aggressiveness of colorectal cancer (CRC) cells and might serve as potential prognostic factors and therapeutic targets. In this study, we therefore globally profiled microRNAs associated with aggressive growth in CRC cells, in an attempt to identify novel prognosti...
ORGANISM(S): Homo sapiens 
2014-01-13 | E-GEOD-53095 | biostudies-arrayexpress
TranscriptomicsmRNA profiling of MEST knockdown in human adipocyte precursor cells at early stage (day 3) of adipocyte differentiation
The Mesoderm-specific transcript homolog protein MEST is a member of the alpha/beta hydrolase fold family that is expressed in adipose tissue, adipocytes as well as their precursor cells. To get an idea about potential functions of MEST in adipocyte development, we knocked down MEST in human Multipo...
ORGANISM(S): Homo sapiens 
2015-06-24 | E-GEOD-63132 | biostudies-arrayexpress
TranscriptomicsmRNA profiling of white hMADS cells converted in brite adipocytes and transfected with mimic miR-125b-5p
miR-125b-5p is a well known miRNA already describded in several forms of cancer. miR-125b-5p is expressed in adipose tissue, adipocytes as well as their precursor cells. We aim to invest the role of miR-125b-5p in white adipocytes conversion into brite adipocytes. To get an idea about putative tar...
ORGANISM(S): Homo sapiens 
2016-07-01 | E-GEOD-80816 | biostudies-arrayexpress
TranscriptomicsIdentification of microRNAs specific for high producer CHO cell lines
Mature microRNA levels were analyzed in 4 producing CHO cell lines expressing 2 structurally different recombinant proteins at low and high level as well as in 1 non-producing CHO cell line. The goal was to identify microRNAs that are involved in heterologous protein formation and secretion. 5 recom...
ORGANISM(S): Cricetulus griseus 
2014-08-04 | E-GEOD-57023 | biostudies-arrayexpress
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