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Using a combination of BONCAT technique and iTRAQ 4-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the h...
ORGANISM(S): Trypanosoma brucei 
2019-12-03 | PXD014580 | Pride
Using a combination of mass spectrometry-based quantitative proteomics, chemical and metabolic labelling, we provide the first protein-level evidence that the Hsp90 inhibition affects the expression of hundreds of proteins in Leishmania spp. Our study defines the Leishmania parasite’s response to Hs...
ORGANISM(S): Leishmania mexicana 
2021-04-23 | PXD024764 | Pride
Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.
ORGANISM(S): Rhizobium leguminosarum 
Time course of bacteroid formation. Bacteroids were harvested from pea nodules at 7, 15 and 21 d after inoculation onto 7 d old pea plants. The transcription profile of each bacteroid sample at the 3 time points was compared to cells grown in free-living culture on succinate ammonia AMS.
ORGANISM(S): Rhizobium leguminosarum 
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