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ProteomicsLFQ of Spot-Trap IP-MS from MEF cells expressing Irgb6-Spot
Irgb6-deficient MEFs stably expressing Spot-tagged Irgb6 were infected with or without T. gondii ME49 for 4 h and lysed in guanidine buffer (6 M guanidine-HCl, 100 mM Tris-HCl, pH 8.0, and 2 mM DTT). The lysates were diluted 8-fold with RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM EGTA, ...
ORGANISM(S): Mus Musculus (mouse) 
2023-11-21 | PXD043421 | JPOST Repository
ProteomicsPolysulfur-based bulking of dynamin-related protein 1 prevents ischemic sulfide catabolism and heart failure
The presence of redox-active molecules containing catenated sulfur atoms (supersulfide) in living organisms has led to a review of the concepts of redox biology and its translational strategy. Glutathione (GSH) is the body’s primary detoxifier and antioxidant, and its oxidized form (GSSG) has been c...
ORGANISM(S): Rattus norvegicus (Rat) 
2024-11-16 | PXD053562 | Pride
Mitochondria Glutathionylation Heart failure Drp1 Supersulfide catabolism 
ProteomicsWhole-cell proteome of Thermosulfidibacter takaii strain ABI70S6
Identification of the proteins from tryptic digests of Thermosulfidibacter takaii strain ABI70S6 whole-cell proteome followed by nanoLC/OrbitrapFusion MS analysis.
ORGANISM(S): Cellular Organisms 
2017-11-24 | PXD007930 | JPOST Repository
ProteomicsAtPhot2, Autophosphorylation, Gel
Recombinant Arabidopsis phototropin2 (Phot2) protein or kinase-dead derivative (Phot2-DN) were incubated with ATP under different conditions. Proteins were separated by SDS-PAGE, in-gel tryptic digested, analyzed by LC-MS.
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2018-10-09 | PXD007908 | JPOST Repository
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