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TranscriptomicsSAGE libraries derived from Bombyx ovaries
These two 3' Long SAGE libraries were constructed from stage 1-3 or stage 2-3 Bombyx egg chambers. Keywords: 3' Long SAGE libraries These libraries were constructed as described in Saha et al, 2002.
ORGANISM(S): Bombyx mori 
2006-10-31 | E-GEOD-5830 | biostudies-arrayexpress
ProteomicsLFQ of GFP-Trap IP-MS of BmN-4 cells expressing BmMasc-GFP
BmMasc-GFP, BmMascNLS-GFP, or GFP was transiently expressed in BmN-4 cells seeded in 10 cm-diameter culture dishes. At three days post transfection, the cells were fixed with 0.1% formaldehyde, washed, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH (pH 7.5), 1 mM EGTA, 1 mM MgCl...
ORGANISM(S): Cellular Organisms 
2025-05-31 | PXD060754 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS of OfMasc-interacting proteins
OfMasc-GFP derivatives or GFP were transiently expressed in OfT1C/tet cells (3.2e6 cells) seeded in 10 cm-diameter culture dishes. At two days post transfection, the cells were fixed, collected, and lysed on ice for 10 min in 1 mL of RIPA buffer (20 mM HEPES-KOH, pH 7.5, 1 mM EGTA, 1 mM MgCl2, 150 m...
ORGANISM(S): Cellular Organisms 
2025-10-25 | PXD059481 | JPOST Repository
ProteomicsLFQ of immunoprecipitates with GFP-Trap from GFP-, OfMasc-GFP-, or Del1-OfMasc-GFP-transfected cells
OfMasc-GFP derivatives or GFP were transiently expressed in OfTC cells. At 2 days post transfection, OfTC cells were treated with 100 nM Bortezomib for 1 day. The OfT1C cells were then fixed with 0.1% formaldehyde, and the fixation was quenched with 300 mM glycine. The cells were lysed on ice in 1 m...
ORGANISM(S): Cellular Organisms 
2022-10-04 | PXD034837 | JPOST Repository
ProteomicsMass spectrometry analysis of venom gland components of the parasitoid wasp Asobara japonica
(1) Secreted materials from the venom gland: 50 venom glands were cultured in 20 μL PBS for 2 hours. The cultured media were collected. Three biological replications were analyzed. (2) Lysates of the venom glands: 50 venom glands were squashed in 50 μL PBS and centrifuged. The supernatants were coll...
ORGANISM(S): Cellular Organisms 
2025-05-07 | PXD052039 | JPOST Repository
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