Biotinylated proteins were enriched with streptavidin beads followed by on-bead trypsin digestion. Then the LC-MS/MS analysis was performed to identify the biotinylated proteins.
First, Flag-FOXQ1 was overexpressed in HCT-116 cells. Subsequently, the Flag-FOXQ1 complex was enriched via immunoprecipitation (IP) technology. The complex was then washed four times with phosphate-buffered saline (PBS), denatured in loading buffer by boiling, and separated via gel electrophoresis ...
