Sort   by:  
 Page size 
TranscriptomicsEffect of FadR overexpression in an engineered fatty acid overproducer
Transcriptome analysis of strains LT-ΔfadE (control) and FRT-ΔfadE (FadR overexpressor) after 12h of fatty acid production. The strains used are described in detail in Zhang F, M Ouellet, TS Batth, CJ Petzold, A Mukhopadhyay and JD Keasling. Enhancing fatty acid production by the expression of the r...
ORGANISM(S): Escherichia coli 
2012-01-28 | E-GEOD-35392 | biostudies-arrayexpress
TranscriptomicsArtemisinic Acid Production Stress in Yeast
We have enginereed S. cerevisiae to produce high titers of Artemisinic acid, an anti-malarial drug precursor. Here we compare the gene expression profiles of the producer strain (EPY330) and a strain in which the last enzyme of the pathway (CYP71AV1, AMO) was inactivated by a point mutation (EPY338)...
ORGANISM(S): Saccharomyces cerevisiae 
2008-10-15 | E-GEOD-11620 | biostudies-arrayexpress
TranscriptomicsCell wide response to low oxygen exposure in Desulfovibrio vulgaris Hildenborough
We investigated the effects of low level oxygen on Desulfovibrio vulgaris Hildenborough using microarrays. Keywords: time course, stress response Comparison of cells treated with 1000 ppm oxygen to untreated cells at 60,120,240 min.
ORGANISM(S): Desulfovibrio vulgaris str. Hildenborough 
2010-06-10 | E-GEOD-7816 | biostudies-arrayexpress
ProteomicsMassively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism
The lysine metabolism of Pseudomonas putida can produce multiple important commodity chemicals and is implicated in rhizosphere colonization. However, despite intensive study, the biochemical and genetic links between lysine metabolism and central metabolism remains unresolved in P. putida. Here, we...
ORGANISM(S): Pseudomonas Putida Kt2440 
PXD011350 | panorama
TranscriptomicsComparison of Labeling Methods for gene expression analysis on the NimbleGen platform
Comparison of ds-cDNA, Indirect and Direct Random Labeling Methods for gene expression analysis on the NimbleGen platform. Expression profiles from artemisinic acid-producing S. cerevisiae strain EPY330 and non-producing strain EPY338 are compared for each labeling method tested. The labeling metho...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-19 | E-GEOD-15816 | biostudies-arrayexpress
ProteomicsExploring Non-Canonical Terpene Space With a Heterologous Lepidopteran Mevalonate Pathway
Lepidoptera (butterflies and moths) make the six-carbon compounds homoisopentenyl pyrophosphate (HIPP) and homodimethylallyl pyrophosphate (HDMAPP) that are incorporated into sixteen, seventeen and eighteen carbon farnesyl pyrophosphate (FPP) analogues. In this work we heterologously expressed the l...
ORGANISM(S): Escherichia Coli 
2019-10-29 | PXD012697 | panorama
ProteomicsAn automated ‘cells-to-peptides’ sample preparation workflow for high-throughput, quantitative proteomic assays of microbes
Mass spectrometry–based quantitative proteomic analysis has proven valuable for clinical and biotechnology related research and development. Driving this value have been improvements in the sensitivity, resolution, and robustness of mass analyzers. However, manual sample preparation protocols are of...
ORGANISM(S): Escherichia Coli Rhodotorula Toruloides Pseudomonas Putida Kt2440 Saccharomyces Cerevisiae 
PXD014182 | panorama
ProteomicsProgrammable polyketide biosynthesis platform for production of aromatic compounds in yeast
To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms are required. Here we present a novel yeast platform for bi...
ORGANISM(S): Saccharomyces Cerevisiae 
2020-02-11 | PXD013388 | panorama
ProteomicsA microbial supply chain for production of anti-cancer drug vinblastine
Monoterpene indole alkaloids (MIAs) are a diverse family of complex plant secondary metabolites with many medicinal properties, including the essential anti-cancer therapeutics vinblastine and vincristine. As MIAs are difficult to chemically synthesize, the world’s supply chain for vinblastine relie...
ORGANISM(S): Saccharomyces Cerevisiae 
2022-09-09 | PXD024976 | panorama
Sort   by:  
 Page size