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ProteomicsIdentification of novel glycan modification on S-layer protein from Methanoculleus Marisnigri
Mass spectrometry and NMR were used to characterize the N- and O-glycan modifications expressed by Methanoculleus marisnigri, a mesophilic methanogen from the Order Methanomicrobiales. The S-layer protein was identified as a PGF-CTERM sorting domain-containing protein encoded by MEMAR_RS02690 and is...
ORGANISM(S): Methanoculleus marisnigri JR1 Archaea 
2023-03-23 | PXD030331 | Pride
O-glycosylation S-layer protein Archaea N-glycosylation Post-translational modification 
TranscriptomicsTargeting MYCN in Neuroblastoma by BET Bromodomain Inhibition
Bromodomain inhibition comprises a promising therapeutic strategy in cancer, particularly for hematologic malignancies. To date, however, genomic biomarkers to direct clinical translation have been lacking. We conducted a cell-based screen of genetically-defined cancer cell lines using a prototypica...
ORGANISM(S): Homo sapiens 
2013-02-25 | E-GEOD-43392 | biostudies-arrayexpress
no omics definedTranscription profiling of T. brucei treated with dithiothreitol (DTT) or tunicamycin to invoke the unfolded protein response
Transcriptome responsiveness was further tested by attempts to invoke the unfolded protein repsonse (UPR), a classic ER-based pathway stimulated by the presence of increased levels of unfolded polypeptides. The UPR is mediated via transcriptional responses in both yeast and metazoan cells, and can b...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1533 | biostudies-arrayexpress
MetabolomicsTargeted metabolomic analysis in hepatic extracts, serum, and urine of female mice gavaged with TCDD every 4 days for 28 days
TCDD is an environmental contaminant that elicits a number of hepatic effects including fat accumulation, inflammation, and fibrosis that can progress to hepatocellular carcinoma. RNA-Seq and targeted metabolomics were integrated with complementary dioxin response element (DRE) location and aryl hyd...
2017-02-02 | MTBLS225 | MetaboLights
TranscriptomicsTranscription profiling of T. brucei bloodstream forms cultured in different proportions of fetal bovine serum to investigate iron-sensing mechanisms
Bloodstream trypanosomes are sensitive to iron starvation, and upregulate ESAG6/7 transferrin receptor (TfR) mRNA and protein levels when placed in low transferrin or iron-depleted media, and adjust TfR expression to compensate for reduced endocytosis. Both observations suggest a specific transcript...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1530 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of T. brucei in vivo or in vitro samples, with in vitro samples either inclubated at 4oC or not to investiage the influence of culturing condtions on intracellular transport
The vast majority of molecular studies on T. brucei are carried out using in vitro cultures. Recently, whole-genome microarray analysis indicated significant differences between in vivo and in vitro cultures of the malaria parasite Plasmodium falciparum. Therefore, as an additional investigation int...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1540 | biostudies-arrayexpress
TranscriptomicsRNAi knock-down of VSG in T. brucei for 1 or 3 days
As an additional strategy for investigating T. brucei transcriptome responsiveness, the mRNA of a highly important protein, the variant surface glycoprotein (VSG) the major surface protein in the bloodstream stage, was suppressed with RNAi. VSG RNAi results in arrest of cell cycle progression...
ORGANISM(S): Trypanosoma brucei 
2008-06-26 | E-MEXP-1528 | biostudies-arrayexpress
ProteomicsRapid, One-step Sample Processing for Label-Free Single-Cell Proteomics
Sample preparation for single-cell proteomics is generally performed in a one-pot workflow with multiple dispensing and incubation steps. These hours-long workflows can be labor intensive and lead to long sample-to-answer times. Here we report a sample processing method that achieves cell lysis, pro...
ORGANISM(S): Homo sapiens (Human) 
2023-10-09 | PXD041879 | Pride
Sample preparation Single-cell proteomics Lc-msms 
UnknownIdentification of a novel N-linked glycan on the archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus.
Not available
S-EPMC7586221 | biostudies-literature
ProteomicsEasy and Accessible Workflow for Label-Free Single-Cell Proteomics
Single cell proteomics (SCP) can provide information that is unattainable through either bulk-scale protein measurements or single-cell profiling of other omes. Maximizing proteome coverage often requires custom instrumentation, consumables and reagents for sample processing and separations, which ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-09 | PXD043355 | Pride
Exploris 480 Hp d100 single cell dispenser Single-cell proteomics 
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