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TranscriptomicsImpact of feeding strategies on the scalable expansion of human pluripotent stem cells in single-use stirred tank bioreactors
Comparison of gene expression profile of human pluripotent stem cells (clone hCBiPS2) expanded in batch-fed and perfusion stirred bioreactors after 0, 3 and 7 days.
ORGANISM(S): Homo sapiens 
2016-08-22 | E-MTAB-3898 | biostudies-arrayexpress
TranscriptomicsEBIO does not induce cardiomyogenesis in human pluripotent stem cells but modulates cardiac subtype enrichment by lineage-selective survival
Human embryonic stem cell (HES3) aggregates were differentiated into cardiomyocytes using biphasic WNT pathway modulation (CHIR99021 and IWP2) in 20mL scale using agitated Erlenmeyer Flask. On day 6 of differentiation, cells were treated with 1mM 1-EBIO or solvent control (DMSO) for 10 days.
ORGANISM(S): Homo sapiens 
2017-01-04 | E-MTAB-5192 | biostudies-arrayexpress
TranscriptomicsControlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture
comparison of human pluripotent stem cell aggregates from Batch (BA) and Cyclic-Perfusion (CPAs) feeding generated in stirred bioreactors.
ORGANISM(S): Homo sapiens 
2014-11-30 | E-MTAB-2814 | biostudies-arrayexpress
TranscriptomicsModulation of the bulk cell density as decisive parameter and simple method for mesendodermal patterning of human pluripotent stem cells
1 million human pluripotent cells cultured as suspension aggregates (hES3-NKX2.5 cell line) were treated with 0, 7.5 or 15µM CHIR99021 in 1 or 3 ml RPMI/B27 (w/o insulin) for 24h to induce the differentiation of human pluripotent stem cells (hPSCs). CHIR99021 is a potent GSK3 inhibitor that results ...
ORGANISM(S): Homo sapiens 
2016-12-30 | E-MTAB-5051 | biostudies-arrayexpress
TranscriptomicsMicroarray of iPSC-derived macrophages generated in a bioreactor process compared to PBMC-derived macrophages and exposed to P. aeruginosa
Human iPSC-Mac or PBMC-mac were seeded on 24-well plates (500.000cells/well) and cultured over night. On the next day, cells were washed three times with PBS. Subsequently, P. aeruginosa laboratory strain PAO1 in RPMI medium without antibiotics (MOI10) was centrifugated on the cells (600xg) and incu...
ORGANISM(S): Homo sapiens 
2018-10-23 | E-MTAB-5436 | biostudies-arrayexpress
UnknownA prognostic model for use before elective surgery to estimate the risk of postoperative pulmonary complications (GSU-Pulmonary Score): a development and validation study in three international cohorts.
Not available
S-EPMC12056793 | biostudies-literature
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