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MetabolomicsDynamic utilization of low-molecular-weight organic substrates across a microbial growth rate gradient

AIM: Low-molecular-weight organic substances (LMWOSs) are at the nexus between micro-organisms, plant roots, detritus and the soil mineral matrix. The nominal oxidation state of carbon (NOSC) has been suggested as a potential parameter for modelling microbial uptake rates of LMWO...

2023-08-14 | MTBLS3558 | MetaboLights
MetabolomicsMapping the metabolism of five amino acids in bloodstream form Trypanosoma brucei using U-13C-labelled substrates and LC–MS
The metabolism of the parasite Trypanosoma brucei has been the focus of numerous studies since the 1940s. Recently it was shown, using metabolomics coupled with heavy-atom isotope labelled glucose, that the metabolism of the bloodstream form parasite is more complex than previously thought. The pres...
2019-06-24 | MTBLS624 | MetaboLights
ProteomicsIn Cellulo Kinase Screen Identifies Novel MASTL Substrates
MASTL (microtubule-associated serine/threonine kinase-like) has emerged as a critical regulator of mitosis and as a potential oncogene in a variety of cancer types. To date, Arpp-19/ENSA are the only known substrates of MASTL. With the cellular roles of MASTL expanding and increased interest in deve...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD028678 | Pride
Akt Phospho-peptides Silac Agc kinase Kinase Mastl 
ProteomicsHigh-throughput identification of FLT3 wild-type and mutant kinase substrate preferences and application to design of sensitive in vitro kinase assay substrates
Abnormalities in the FLT3 signaling pathway play an integral role in AML disease relapse and drug resistance. Developing new and specific FLT3 tyrosine kinase inhibitors for use in combination to induction therapy is an important step to reduce disease relapse and achieve clinical remission. To deve...
ORGANISM(S): Homo sapiens (Human) 
2019-01-07 | PXD011478 | Pride
Kinase assay linked with phosphoproteomics Flt3 Acute myeloid leukemia Mass spectrometry Phosphoproteomics 
ProteomicsIdentifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Mass spectrometry‐based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically‐altered enzymecofactor systems. We describe a method that combines stable γ‐[18O2]‐ATP with classical in v...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD002133 | Pride
Human Kinase substrates  in vitro kinase assay  proteomics  mass spectrometry  heavy atp  non receptor tyrosine kinase abl1 
ProteomicsIdentification of CDK1 substrates in mitosis by quantitative phosphoproteomics
Cyclin-dependent kinase 1 (Cdk1) is an essential regulator of many mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. Deregulation of Cdk1 activity results in defects in these processes. Although the role of Cdk...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-02-24 | MSV000079528 | MassIVE
ProteomicsKinase-catalyzed Biotinylation for Discovery and Validation of Substrates to Multi-specificity Kinases NME1 and NME2
Protein phosphorylation by kinases regulates mammalian cell functions, such as growth, division, and signal transduction. Among human kinases, NME1 and NME2 are associated with metastatic tumor suppression, but remain understudied due to the lack of tools to monitor their cellular substrates. In par...
ORGANISM(S): Homo sapiens (Human) 
2024-10-17 | PXD051411 | Pride
Proteomics Histidine kinase Phosphorylation Nme Substrate specificity Atp-biotin Protein kinase 
ProteomicsProteomic profiling by array of tyrosine kinase activity in metastatic malignant melanoma from human tissue specimens
Tyrosine kinase activity profiling of metatstatic malignant melanoma and normal skin tissue samples was performed using peptide kinase arrays. All samples were run in triplicates with and withouth inhbitor PLX4032 and Sutent Malate in order to identify how tyrosine kinases responds to kinase inhibit...
ORGANISM(S): Homo sapiens 
2013-01-17 | E-MTAB-1245 | biostudies-arrayexpress
ProteomicsThe innate immunity kinase TAK1 phosphorylates Rab1 on a hotspot for post-translational modifications by host and pathogen
TGF-β Activated Kinase 1 (TAK1) is a critical signaling hub responsible for translating antigen binding signals to immune receptors for the activation of the AP-1 and NF-κB master transcriptional programs. Despite its importance, known substrates of TAK1 are limited to kinases of the MAPK and IKK fa...
ORGANISM(S): Homo sapiens (Human) 
2016-07-19 | PXD004213 | Pride
Kinase Covalent capture Kinase substrates Analog specific kinase 
ProteomicsSubstrate selectivity for semisynthetic CK2 proteins with various posttranslational modifications
Protein Ser/Thr kinase CK2 is involved in a myriad of cellular processes including cell growth and proliferation by phosphorylating hundreds of substrates, yet the regulation process of CK2 function is poorly understood. The CK2 catalytic subunit, CK2α, is modified by O-GlcNAc on Ser347 proximal to...
ORGANISM(S): synthetic construct 
2011-10-24 | E-GEOD-33149 | biostudies-arrayexpress
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