Sort   by:  
 Page size 
ProteomicsMDM2 influenced chromatin-associated phospho-peptides in T47D cells
Employing a stable isotope labeling in cell culture analysis in T47D breast cancer cells (expressing mtp53 L194F), we uncovered several chromatin-associated DNA replication and repair factors as MDM2-regulated (by MDM2 inducible knock-down) phosphoproteins, including 53BP1
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD061454 | Pride
Chromatin-associated Phospho-peptides Mdm2 53bp1 
UnknownEnhancing Top-Down Analysis Using Chromophore-Assisted Infrared Multiphoton Dissociation from (Phospho)peptides to Protein Assemblies.
Not available
S-EPMC7711774 | biostudies-literature
ProteomicsThe Arabidopsis tyrosine phosphatase RLPH2 targets D-group MAP kinases
Knowing that biochemically RLPH2 is a tyrosine phosphatase, we selectively In an attempt to discover RLPH2 substrates we decided to use the power of phospho-proteomics. Immunoprecipitated tyrosine phosphorylated peptides from enriched phospho-peptide pools derived from these plant extracts. Mass spe...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-01-26 | PXD038689 | Pride
Phosphoproteomics Arabidopsis Protein phosphatase Mapk 
ProteomicsHiRIEF LC-MS allows for in depth quantitative analysis of the phosphoproteome.
In this project we aimed at improving phosphoproteomics analysis by taking advantage of high resolution isoelectric focusing (HiRIEF) fractionation. We developed a workflow that employs titanium dioxide phospho-enrichment, followed by isobaric labeling with Tandem Mass Tags (TMT) and HiRIEF on a bro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-07 | PXD005410 | Pride
Multiplex Cells Isoelectric point Tmt Phospho-peptides Phospho-sites Fractionation Quantification Networkin Cancer Phosphoproteomics Tyrosine Ipg Proteomics Hirief 10-plex Lc-ms Quantitative Mitosis Mitotic arrest Hela Kinase association Multiply phosphorylated Phosphorylation Pervanadate 
ProteomicsPhosphorylation of Asc1p and Asc1p-dependent phospho-proteome
Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome We determined 1) the phosphorylation sites within the Asc1 protein purified from Saccharomyces cerevisiae via its Strep-tag and 2) the Asc1p-dependent phospho-proteome. 1) Phospho-sites within tryptic peptides of Asc1p were identified usi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-12-13 | PXD003031 | Pride
Lc-msms Asc1p Rack1 Silac Signal transduction Phosphorylation Saccharomyces cerevisiae Translation 
ProteomicsA compendium of murine (phospho)peptides encompassing different isobaric labeling and data acquisition strategies
Targeted mass spectrometry-based assays typically rely on previously acquired large datasets for peptide target selection. Such repositories are widely available for unlabeled peptides. However, they are less common for isobaric tagged peptides. Here we have assembled two series of six datasets orig...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-03 | PXD024298 | Pride
Faims Tmtpro Tmt Targeted proteomics Eclipse 3t3 
ProteomicsStructure-guided development of chemically tailored peptide binders of RNF43/ZNRF3 to enable versatile design of membrane protein-targeting PROTACs
Targeted membrane protein degradation using cell surface E3 ligases RNF43/ZNRF3 via proteolysis targeting chimeras (PROTACs) represents an effective strategy for treating membrane drug targets that cannot be fully inhibited using traditional inhibitors. Several ingenious chimeras have been developed...
ORGANISM(S): Homo Sapiens 
2025-03-04 | PXD061425 |
ProteomicsCharacterisation of proteome of a novel Escherichia coli strain exhibiting mucoviscous phenotype.
Protein expression by E. coli 26561 during the late-exponential phase of cultures under anaerobic conditions was examined. E. coli 26561 is a multidrug resistant (MDR) and shows an unusual hyper-mucoviscous phenotype. Resistance includes ESBL (CTX-M-14) and proteome was determined with and without...
ORGANISM(S): Escherichia coli 
2018-08-22 | PXD008339 | Pride
Anaerobic Ceftazidime Tmt Escherichia coli Antibiotic Mucoviscous Mic Shotgun Quantification 
ProteomicsTruncation of the N-terminus of cardiac troponin I initiates adaptive remodeling of the myocardial proteosome via phosphorylation of mechano-sensitive signaling pathways
We employed isobaric labeled peptides to do bottom-up proteomics to quantify both non-enriched total peptides and enriched phospho-peptides obtained from hearts of control mice expressing wild-type cardiac troponin I and transgenic mice expressing the truncated N-terminal cardiac troponin I.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-03-15 | MSV000087049 | MassIVE
ProteomicsNovel antibody-peptide binding assay indicates presence of immunoglobulins against EGFR phospho-site S1166 in high-grade glioma.
The project’s aim was to develop and test the applicability of a method to determine the presence of serum immunoglobulins against specific autoantibodies. The method combined immunoglobulin G enrichment (using Melon Gel), molecular weight filtration, and targeted mass spectrometry and the feasibili...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2022-06-09 | PXD032844 | Pride
Glioblastoma Melon gel Peptide antigen Autoantibodies Immunoglobulin g 
Sort   by:  
 Page size