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ProteomicsHiRIEF LC-MS allows for in depth quantitative analysis of the phosphoproteome.
In this project we aimed at improving phosphoproteomics analysis by taking advantage of high resolution isoelectric focusing (HiRIEF) fractionation. We developed a workflow that employs titanium dioxide phospho-enrichment, followed by isobaric labeling with Tandem Mass Tags (TMT) and HiRIEF on a bro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-07 | PXD005410 | Pride
Multiplex Cells Isoelectric point Tmt Phospho-peptides Phospho-sites Fractionation Quantification Networkin Cancer Phosphoproteomics Tyrosine Ipg Proteomics Hirief 10-plex Lc-ms Quantitative Mitosis Mitotic arrest Hela Kinase association Multiply phosphorylated Phosphorylation Pervanadate 
TranscriptomicsRNA-seq of A431 cell line after gefitinib treatment
RNA sequencing of A431 cell line samples before and after gefitinib treatment, at 0, 2, 6 and 24 hours, was performed in order to characterize the cell line's early and late response to this drug, and to compare against proteomics (mass spectrometry) characterization of the cell line using the same ...
ORGANISM(S): Homo sapiens 
2016-12-01 | E-MTAB-5285 | biostudies-arrayexpress
ProteomicsHiRIEF TMT Proteomics of Arabidosis thaliana, wildtype versus oligopeptidases' KO mutants
Oligopeptidases Prep and OOP are enzymes located in mitochondria and chloroplasts responsible for the cleavage of long peptides (8 to 65 aas) down to short peptides (3 to 7 aas), which in turn are degraded to free amino acids by aminopeptidases such as M1 and M17. In this study, Arabidopsis thalian...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-11-14 | PXD009651 | Pride
Arabidopsis thaliana Proteomics Tmt Prep Oligopeptidase Hirief Lcms Oop 
ProteomicsHiRIEF deep proteomics of A431 human vulvar cancer cell line and N2A mouse neuroblastoma cell line
Here we present high resolution data from two cancer cell lines. A human cell line, A431 was treated with a drug at different time points and then fractionated into its different subcellular compartments (nuclear, organellar, soluble,whole cell). Protein extracts were trypsinized, peptides separated...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2013-09-27 | PXD000065 | Pride
Mouse Lc-ms Hirief Isoelectric focusing Cancer N2a Human A431 
UnknownIsoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome.
Not available
S-EPMC5495806 | biostudies-literature
ProteomicsProteomic profiling of glioblastoma tumour tissue
Using HiRIEF LC-MS/MS, we analysed 10 GBM tumour tissue samples ran in one TMT10 set. The set consisted of 7 primary tumours and 3 non-matched recurrent tumours. One primary tumour was highly necrotic.
ORGANISM(S): Homo sapiens (Human) 
2022-12-23 | PXD027341 | Pride
Tissue Glioblastoma Brain cancer Hirief Lc-ms/ms 
ProteomicsMouse HiRIEF LCMS Proteomics of liver tissue - WT VS PolgA KO mutant
Mitochondrial dysfunction is implicated in aging and aging-related disorders, such as neurodegenerative diseases and stroke. To study the effects of progressive mitochondrial dysfunction, a homozygous knock-in mouse expressing a proof-reading deficient version of the nucleus-encoded catalytic subuni...
ORGANISM(S): Mus musculus (Mouse) 
2025-02-05 | PXD047574 | Pride
Mouse Tmt10plex Liver Wild type Hirief Lcms Murine Polga knockout Wt Qexactive 
ProteomicsProteomic profiling of glioblastoma stem cells - HGCC cohort
With HiRIEF LC-MS/MS, we analysed 11 GBM stem cell lines from the HGCC cohort (Uppsala, Sweden). The samples were run in one TMT16 set, with three samples analysed in duplicates, and the last two channels left empty.
ORGANISM(S): Homo sapiens (Human) 
2022-12-23 | PXD027339 | Pride
Glioblastoma Stem cells Hirief Brain cancer Cell line Lc-ms/ms 
ProteomicsHiRIEF Proteomics of Arabidopsis thaliana Col-0 and CIAF1 mutants
The assembly of the mitochondrial Complex I requires the expression of nuclear and mitochondrial located genes, co-factor biosynthesis and the assembly of at least 44 subunits. This requires the involvement of assembly factors that interact with subunits of Complex I but are not part of the final ho...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-10-08 | PXD011795 | Pride
Arabidopsis thaliana Salk_143656 Mitochondria Tim23 Complex i At1g76060 Hirief Ciaf1 Col-0 
ProteomicsLabel free HiRIEF deep proteomics of A431 human vulvar cancer cell line.
Human A431 cells were lysed. Protein extracts were trypsinized, peptides separated by HiRIEF (high resolution isoelectric focusing) and analysed by LC-MS.
ORGANISM(S): Homo sapiens (Human) 
2019-12-16 | PXD000542 | Pride
Lc-ms Hirief Isoelectric focusing Cancer Human A431 
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