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MetabolomicsIntegrating two chromatographies in a co-fractionation mass spectrometry approach improved protein-metabolite interactions (PMIs) mapping capabilities and revealed novel protein-ligand pairings in Escherichia coli.
The protein-metabolite interactome remains understudied. We previously developed a co-fractionation mass-spectrometry-based approach called PROMIS to address this gap. PROMIS relies on size separation chromatography of protein-metabolite complexes from lysates, followed by proteomics and metabolomic...
2025-04-25 | MTBLS9098 | MetaboLights
GenomicsLignocellulose fractionation
Lignocellulose fractionation by microbial consortia
PRJEB26853 | ENA
TranscriptomicsBiochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA
Biochemical fractionation of HEK293 nuclei and RNA-seq of chromatin-associated and soluble-nuclear RNA. Nuclei from three biological replicates were isolated by detergent lysis, fractionated, then three chromatin and three soluble RNA samples were converted to cDNA using Illumina TruSeq stranded pro...
ORGANISM(S): Homo sapiens 
2015-03-04 | E-GEOD-66478 | biostudies-arrayexpress
TranscriptomicsMembrane-polysome associated fractionation of HES2 embryonic stem cells
The membrane fraction and the cytosolic fraction of HES2 cells were collected and subjected to microarray. The experiment was performed in triplicate Keywords: membrane-polysome fractionation of RNA Three consecutive passages of HES2 cells were grown and subject to membrane-polysome fractionation....
ORGANISM(S): Homo sapiens 
2010-04-07 | E-GEOD-14037 | biostudies-arrayexpress
ProteomicsComparison of fractionation proteomics for local SWATH library building
For data-independent acquisition by means of sequential window acquisition of all theoretical fragment ion spectra (SWATH), a reference library of data-dependent acquisition (DDA) runs is typically used to correlate the quantitative data from the fragment ion spectra with peptide identifications. Th...
ORGANISM(S): Homo sapiens (Human) 
2017-07-04 | PXD006190 | Pride
Library building Comparative study Swath-ms Fractionation proteomics Hesc 
TranscriptomicsGenomewide demarcation of RNA PolII transcription units by physical fractionation of chromatin- ORF enrichment
Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the ot...
ORGANISM(S): Saccharomyces cerevisiae 
2006-08-30 | E-GEOD-5649 | biostudies-arrayexpress
GenomicsRNA-fractionation sequencing
RNA-fractionation sequencing
PRJNA791311 | ENA
ProteomicsIPG Strip-Based Peptide Fractionation for Shotgun Proteomics
This deep proteome dataset of human HEK293 cells was generated using IPG Strip-Based fractionation.
ORGANISM(S): Homo sapiens (Human) 
2015-07-08 | PXD002389 | Pride
Hek293 Ipg fractionation 
GenomicsBiogas isotope fractionation
Isotope fractionation in the biogas allows direct microbial community stability monitoring in anaerobic digestion.
PRJEB20414 | ENA
TranscriptomicsGenomewide demarcation of RNA PolII transcription units by physical fractionation of chromatin- ORF-INT enrichment
Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the ot...
ORGANISM(S): Saccharomyces cerevisiae 
2006-08-30 | E-GEOD-5650 | biostudies-arrayexpress
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