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ProteomicsHiRIEF LC-MS allows for in depth quantitative analysis of the phosphoproteome.
In this project we aimed at improving phosphoproteomics analysis by taking advantage of high resolution isoelectric focusing (HiRIEF) fractionation. We developed a workflow that employs titanium dioxide phospho-enrichment, followed by isobaric labeling with Tandem Mass Tags (TMT) and HiRIEF on a bro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-07 | PXD005410 | Pride
Multiplex Cells Isoelectric point Tmt Phospho-peptides Phospho-sites Fractionation Quantification Networkin Cancer Phosphoproteomics Tyrosine Ipg Proteomics Hirief 10-plex Lc-ms Quantitative Mitosis Mitotic arrest Hela Kinase association Multiply phosphorylated Phosphorylation Pervanadate 
UnknownShort and simple sequences favored the emergence of N-helix phospho-ligand binding sites in the first enzymes.
Not available
S-EPMC7071883 | biostudies-literature
GenomicsTLE1 mediates estrogen receptor binding
Estrogen receptor (ER) binds to distal enhancers within the genome and requires additional factors, such as the Forkhead protein FoxA1, for mediating chromatin interactions. We now show that the human Groucho protein, Transducin-like enhancer protein 1 (TLE1), positively assists some ER-chromatin in...
ORGANISM(S): Homo sapiens 
2012-11-14 | E-MTAB-1368 | biostudies-arrayexpress
GenomicsMus musculus
Phospho-HLXB9 binding sites in mouse insulinoma cells
PRJNA261071 | ENA
ProteomicsPhosphorylation of Asc1p and Asc1p-dependent phospho-proteome
Phosphorylation of Asc1p and Asc1p-dependent phospho-proteome We determined 1) the phosphorylation sites within the Asc1 protein purified from Saccharomyces cerevisiae via its Strep-tag and 2) the Asc1p-dependent phospho-proteome. 1) Phospho-sites within tryptic peptides of Asc1p were identified usi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-12-13 | PXD003031 | Pride
Lc-msms Asc1p Rack1 Silac Signal transduction Phosphorylation Saccharomyces cerevisiae Translation 
ProteomicsProteomics of phosphorylation and protein dynamics during fertilization and meiotic exit in the Xenopus egg
Fertilization triggers release from meiotic arrest and initiates events that prepare for the ensuing developmental program. Protein degradation and phosphorylation are known to regulate protein activity during this process. However, the full extent of protein loss and phospho-regulation is still unk...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2017-11-29 | PXD006639 | Pride
Xenopus Egg Fertilization 
GenomicsPhospho-HLXB9 binding sites in mouse insulinoma cells
We have shown that increased β-cell proliferation in functioning pancreatic neuroendocrine tumors (insulinomas) correlated with expression of phosphorylated isoform of the transcription factor HLXB9 which is an embryonic beta-cell differentiation factor (HLXB9 is also known as HB9, MNR2, and MNX1). ...
ORGANISM(S): Mus musculus 
2015-09-30 | GSE61432 | GEO
ProteomicsGas6/Axl signaling activates Akt in liver cancer cells
We investigated which signaling pathways downstream of Gas6/Axl promote the invasive phenotype of liver cancer cells. Thus, we performed phospho-proteomic analysis of MR hepatocytes and those expressing Axl (MR-Axl) in the presence and absence of Gas6. Protein-clustering based on the pattern of phos...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-23 | PXD047577 | Pride
Cell invasion Axl Hepatocellular carcinoma. Gas6 
ProteomicsCharacterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain
In this project we applied a workflow for phosphoproteomics analysis to characterize the phosphorylation signalling that is triggered by the cardiotonic steroid ouabain. Ouabain is a ligand for the sodium-potassium ATPase (NKA). Low concentration of ouabain trigger oscillation of intracellular calci...
ORGANISM(S): Chlorocebus sabaeus 
2019-09-13 | PXD010006 | Pride
Phosphoproteomics Ip3r Akt Mapk Calcium-calmodulin-dependent protein kinase Nka Insp3 receptor Calcium Ouabain Na-k-atpase Sodium-potassium atpase Apoptosis Calcium oscillations Camk 
TranscriptomicsiCLIP Analysis of WT, phospho-defective, and phospho-mimicking mutants of mouse Nucleolin in iKras PDAC cells
This experiment aimed to identify Nucleolin (Ncl) binding sites on a transcriptome-wide scale, by performing iCLIP in mouse iKras PDAC cells (Ying et al., 2012) transiently transfected with a mammalian expression construct encoding a wild-type (WT) myc-Ncl, a phospho-defective mutant in which S28, S...
ORGANISM(S): Mus musculus 
2023-03-01 | E-MTAB-12481 | biostudies-arrayexpress
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