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Target genes of Fbxl10 during 3T3-L1 adipogenesis was analyzed 3T3-L1 cells overexpressing Fbxl10 using retrovirus system containing LTR promoter were differentiated and RNA was extracted at day 2 of differentiation
ORGANISM(S): Mus musculus 
The significant changes of hematopoietic cells induced by Xbp1S expression indicate that there is global alteration in gene expression. UPR induces transcription of Xbp1, and phosphorylation of the ER transmembrane kinase IRE1 initiates UPR-mediated mRNA splicing of Xbp1, resulting in the production...
ORGANISM(S): Mus musculus 
G0 marker-separation of dormant and active hematopoietic stem cells reveals dormancy regulation by basal cytoplasmic calcium
ORGANISM(S): Mus Musculus (mouse) 
In order to explore molecules whose expression is controlled by Slc39a13, we investigated gene expression profiling of primary osteoblast isolated from wild-type and Slc39a13 knockout mice. Keywords: knockout vs wild-type wild-type vs Slc39a13 knockout
ORGANISM(S): Mus musculus 
High levels of Hes1 expression are frequently found in BCR-ABL-positive chronic myelogenous leukemia in blast crisis (CML-BC). In mouse bone marrow transplantation (BMT) models, co-expression of BCR-ABL and Hes1 induces CML-BC–like disease; however the underlying mechanism remained elusive. Here, ba...
ORGANISM(S): Mus musculus 
NIH3T3 in the middle of G0 to G1 transion consists of the cells which is still staying G0 phase and the cells which enters G1. Monitoring the expressions of p27 and Cdt1 enables to distinguish these two; p27+/Cdt1+ cells as the cells in G0 phase and p27-Cdt1+ cells as G1 phase We sorted p27+Cdt1+ (...
ORGANISM(S): Mus musculus 
The cell lysates were mixed with anti-FLAG-antibody-crosslinked Dynabeads. The beads-lysate mix was incubated at 4°C for 45 min. After washing with PBS-T, proteins were eluted with Laemmli’s SDS ample buffer by incubating at 37°C for 30 min. The collected supernatants were frozen at -80°C as samples...
ORGANISM(S): Homo Sapiens (human) 
Evi1 is essential for proliferation of hematopoietic stem cells and implicated in the development of myeloid disorders. Particularly, high Evi1 expression defines one of the largest clusters in acute myeloid leukemia and is significantly associated with extremely poor prognosis. Improvement of the t...
ORGANISM(S): Mus musculus 
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