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TranscriptomicsCellular aging can be monitored by DNA-methylation changes at specific CpG sites
Long-term culture associated changes need to be considered for quality control of cell preparations – especially in cellular therapy. Here we describe a simple method to track cellular aging based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic signature can be used ...
ORGANISM(S): Homo sapiens 
2012-05-21 | E-GEOD-29661 | biostudies-arrayexpress
TranscriptomicsMethylation Pattern of Human Dermal Fibroblasts
In this study, we have analyzed DNA methylation changes upon aging of human dermal fibroblasts by using the HumanMethylation27 BeadChip assessing 27,578 unique CpG sites. Cells were isolated from skin samples donated by young (6-23 years) and elderly (60-73 years) patients undergoing surgical interv...
ORGANISM(S): Homo sapiens 
2012-02-14 | E-GEOD-22595 | biostudies-arrayexpress
TranscriptomicsReplicative senescence of mesenchymal stem cells causes DNA methylation changes which correlate with repressive histone marks
Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long‐term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony‐ forming unit (CFU‐f) frequency and the differentiation potential ...
ORGANISM(S): Homo sapiens 
2011-10-27 | E-GEOD-26519 | biostudies-arrayexpress
TranscriptomicsAffymetrix SNP array data for different passages of mesenchymal stem cells.
Cells in culture undergo replicative senescence and unequivocally stop proliferation after a limited number of cell divisions. In this study, we have expanded mesenchymal stem cells (MSC) from human adipose tissue and analyzed genetic and epigenetic sequels. The subpopulation of highly proliferative...
ORGANISM(S): Homo sapiens 
2013-02-15 | E-GEOD-27135 | biostudies-arrayexpress
TranscriptomicsNID2 and HOXA9 promoter hypermethylation as biomarkers to prevent and detect Oral Cavity Squamous Cell Carcinoma
Differentially methylated oral squamous cell carcinoma (OSCC) biomarkers, identified in-vitro and validated in well-characterized surgical specimens, have shown poor clinical correlation in cohorts with different risk profiles. To overcome this lack of relevance we used the HumanMethylation27 BeadCh...
ORGANISM(S): Homo sapiens 
2013-02-13 | E-GEOD-28217 | biostudies-arrayexpress
GenomicsReplicative senescence is associated with nuclear reorganization and DNA methylation at specific transcription factor binding sites (MBD-seq)
Primary cells enter replicative senescence after a limited number of cell divisions. This process is associated with reproducible changes in DNA methylation (DNAm) at specific sites in the genome. The mechanism that drives senescence-associated DNAm changes remains unknown and may arise through drif...
ORGANISM(S): Homo sapiens 
2015-03-26 | E-GEOD-59960 | biostudies-arrayexpress
TranscriptomicsPluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes [Illumina]
Pluripotent stem cells evade replicative senescence, whereas other primary cells lose their proliferation and differentiation potential after a limited number of cell divisions M-bM-^@M-^S and this is accompanied by specific senescence-associated DNA methylation (SA-DNAm) changes. Here, we investiga...
ORGANISM(S): Homo sapiens 
2012-09-24 | E-GEOD-37066 | biostudies-arrayexpress
TranscriptomicsPluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes
This SuperSeries is composed of the following subset Series: GSE37065: Long-term culture associated gene expression changes in MSC [Affymetrix] GSE37066: Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes [Illumina] GSE38806: Gene expression profiles of induced pluripot...
ORGANISM(S): Homo sapiens 
2012-09-24 | E-GEOD-37067 | biostudies-arrayexpress
UnknownDemographic, clinical and antibody characteristics of patients with digital ulcers in systemic sclerosis: data from the DUO Registry.
Not available
S-EPMC3329234 | biostudies-literature
UnknownVasopressor use after noncardiac surgery: an international observational study.
Not available
S-EPMC12799376 | biostudies-literature
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