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Proteomics2D-LC/MS/MS analysis of Escherichia coli during exposure to abrupt downshifts in temperature and water activity
2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon abrupt downshifts from 35°C aw 0.993 to 14°C aw 0.967. Bacterial cells were harvested before abrupt downshifts in both temperature and aw (i.e. control), or at 0 (i.e. immediately after t...
ORGANISM(S): Escherichia coli 
2016-09-29 | PXD004803 | Pride
Cold temperature 2d-lc/ms/ms Water activity stress E. coli 
Proteomics2D-LC/MS/MS analysis of Escherichia coli during exposure an abrupt downshift in water activity
2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon an abrupt downshift in water activity (aw) (i.e., from aw 0.993 to aw 0.967) at a constant temperature of 35°C. Bacterial cells were harvested before hyperosmotic shift, and 0 (i.e. immed...
ORGANISM(S): Escherichia coli 
2014-04-14 | PXD000541 | Pride
Physiology Stress response 2d-lc/ms/ms Water activity stress E. coli 
Proteomics2D-LC/MS/MS analysis of Escherichia coli during exposure to an abrupt temperature downshift
2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon an abrupt downshift in temperature (i.e., from 35°C to 14°C). Cell cultures were harvested at 30, 90, 160, and 330 min post-temperature downshift. It also should be noted that these time ...
ORGANISM(S): Escherichia coli 
2019-11-25 | PXD000573 | Pride
Physiology Stress response 2d-lc/ms/ms E. coli Cold stress 
Proteomics2D-LC/MS/MS analysis of Escherichia coli under optimal growth condition
2D-LC/MS/MS analysis was used to examine the proteome of E. coli O157:H7 strain Sakai during exponential growth under optimal condition (i.e., from 35°C aw 0.993). MS/MS data obtained from each protein sample were processed by the Computational Proteomics Analysis System (CPAS), a web-based system ...
ORGANISM(S): Escherichia coli 
2019-12-04 | PXD000583 | Pride
Optimal growth condition Physiology 2d-lc/ms/ms E. coli 
TranscriptomicsGlobal genome response of Escherichia coli to temperature downshift
In a previous study we adopted an integrated transcriptomic and proteomic approach to determine the physiological response of E. coli O157:H7 Sakai during exponential phase growth under steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass ...
ORGANISM(S): Escherichia coli 
2014-10-18 | E-MTAB-2010 | biostudies-arrayexpress
TranscriptomicsGlobal genome response of Escherichia coli to water activity downshift
In a previous study we adopted an integrated transcriptomic and proteomic approach to determine the physiological response of E. coli O157:H7 Sakai during exponential phase growth under steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcass ...
ORGANISM(S): Escherichia coli 
2014-10-18 | E-MTAB-2011 | biostudies-arrayexpress
TranscriptomicsMolecular response of Escherichia coli O157:H7 Sakai during dynamic changes in growth kinetics induced by an abrupt downshift in temperature and water activity
This experiment contains the transcriptomic dataset that constitutes part of an integrated transcriptomic and proteomic study monitoring the response of exponential phase E. coli O157:H7 Sakai cultures upon an abrupt downshift in temperature and water activity (from 35°C aw 0.993 to 14°C aw 0.967)...
ORGANISM(S): Escherichia coli 
2016-09-16 | E-MTAB-3969 | biostudies-arrayexpress
TranscriptomicsIntegrated transcriptomic and proteomic analysis of the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions of cold and water activity stress
An integrated genomic and proteomic analysis was undertaken to determine the physiological response of Escherichia coli O157:H7 Sakai to steady-state conditions relevant to low temperature and water activity conditions experienced during meat carcase chilling in cold air. The response of E. coli dur...
ORGANISM(S): Escherichia coli 
2011-12-29 | E-MTAB-623 | biostudies-arrayexpress
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