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Samples were taken from either surgically resected specimens or during surveillance colonoscopic examination. The expression profiles were determined using Affymetrix Human Genome U133 Plus 2.0 arrays. Comparison between the sample groups allow to identify a set of discriminating genes that can be u...
ORGANISM(S): Homo sapiens 
Proximal proteins to S1PR1-WT and S1PR1-TM4 (a swap mutant of the transmembrane domain 4 with that of S1PR3) were obtained in human umbilical vein endothelial cells by a TurboID-catalyzed proximity biotin labeling technique.
ORGANISM(S): Homo Sapiens (human) 
HEK293T cells were cultured in 6-well plates and treated with DMSO or 2 µM MLN4924. After 6 h (MLN4924) or 24 h (DMSO or MLN4924), the cells (n=3) were collected using 1 mL of PBS by pipetting. All samples were centrifuged at 500 × g for 3 min at 4°C, and cell pellets were lysed in 160 µL of guanidi...
ORGANISM(S): Homo Sapiens (human) 
HEK293T cells transfected with control, CUL4, COP1, DCAF3, DCAF14, or RBBP7 siRNA were lysed in 150 µL guanidine buffer. After heating and sonication, the lysates were centrifuged at 20,000 × g for 15 min at 4°C. The supernatants were recovered, and proteins (150 µg each) were purified by methanol-c...
ORGANISM(S): Homo Sapiens (human) 
HEK293T cells stably expressing AGIA-AirID-DCAF, -SKP2, and -IκBα were cultured in a 10-cm dish in biological replicates (n = 2) and treated with 10 µM MG132 for 6 h in the presence of 10 µM biotin for 24 h. The cell pellet was then lysed in 300 µL of a guanidine buffer (6 M guanidine-HCl, 100 mM HE...
ORGANISM(S): Homo Sapiens (human) 
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