Sort   by:  
 Page size 
ProteomicsLFQ of anti-Myc IP-MS of OMA1-Myc-expressing MEFs
OMA1 KO MEFs (10 cm dish) and its rescued cells stably expressing WT or EQ mutant OMA1-Myc were incubated with 0.2% (w/v) formaldehyde for 15 min at 37° C, followed by quenching with 100 mM glycine-NaOH (pH 7.5) for 10 min at room temperature. After once wash with 1× PBS (pH 7.4), the cells were scr...
ORGANISM(S): Mus Musculus (mouse) 
2026-01-31 | PXD063014 | JPOST Repository
ProteomicsLFQ of neo-amino terminal peptides using Tryp-N
To determine candidate OMA1 substrates during mitochondrial stress, OMA1 KO MEFs or its rescued cells expressing OMA1-Myc were treated with or without 40 uM FCCP for 2 h, then washed once with 1× PBS (pH 7.4), and cell pellets were collected. The pellets were then lysed in a guanidine buffer [6 M gu...
ORGANISM(S): Mus Musculus (mouse) 
2026-01-31 | PXD063017 | JPOST Repository
ProteomicsLFQ of anti-Myc IP-MS of AIFM1(TCS) or AIFM1(TCS/TEV)-expressing cells
The mitochondrial fractions from the AIFM1(TCS)- and AIFM1(TCS/TEV)-expressing cells were lysed in 1 mL of lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 10% (w/v) glycerol, 1 mM EDTA, 0.5% (w/v) digitonin, and protease inhibitor cocktail. After centrifugation, the clarified supernata...
ORGANISM(S): Homo Sapiens (human) 
2026-01-31 | PXD063094 | JPOST Repository
ProteomicsDIA-PASEF analysis of lung tissues from influenza-infected and uninfected mice
Lung tissues from uninfected and infected mice (n = 5 per group) were homogenized in 0.5 mL of guanidine buffer. After heating and sonication, the lysates were centrifuged at 20,000 g for 15 min at 4° C. The resulting supernatants were collected, and the proteins (20 µg each) were purified using the...
ORGANISM(S): Mus Musculus (mouse) 
2026-01-31 | PXD069450 | JPOST Repository
Proteomics100uM_DSBU_XL-MS_PHB1-Myc_HEK293
Mitochondria were isolated from HEK293 cells that stably express PHB1 with an C-terminal 3×Myc tag, and were resuspended in a cross-linking buffer [1 × PBS (pH 7.2) and 100 μM disuccinimidyl didutyric urea (DSBU) for 30 min at room temperature. The cross-linked mitochondria were then lysed in RIPA b...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD014217 | JPOST Repository
ProteomicsBioID2_BirA-MAVS_CLPB-BirA_PHB1-BirA_HEK293
HEK293 cells that stably express MAVS, CLPB or PHB1 fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin agarose beads for 2 h at ...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD014218 | JPOST Repository
Proteomics50_100uM_DSBU_XL-MS_PHB2(V1)-Myc_HEK293
Mitochondria were isolated from HEK293 cells that stably express PHB2(V1) with an C-terminal 3×Myc tag, and were resuspended in a cross-linking buffer [1 × PBS (pH 7.2) and 50 or 100 μM disuccinimidyl didutyric urea (DSBU) for 30 min at room temperature. The cross-linked mitochondria were then lysed...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD011941 | JPOST Repository
ProteomicsBioID2_PHB2(V1)_PHB2(V3)-BirA-HA_HEK293
HEK293 cells that stably express PHB2 (V1 or V3) fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin agarose beads for 2 h at 4° ...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD011939 | JPOST Repository
ProteomicsBioID2_PHB2(V1)-(WT_KKK_I225P)
HEK293 cells that stably express wild-type (WT), E229/231/233K (KKK), or I225P mutant PHB2(V1) fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated ...
ORGANISM(S): Homo Sapiens (human) 
2019-09-05 | PXD011946 | JPOST Repository
Sort   by:  
 Page size