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TranscriptomicsATAC-seq experiment in two versions of Raji cells with inducible BZLF1 variants prior to and after induction of EBV's lytic cycle by doxycycline.
The aim of the experiment was to identify accessible (open) chromatin regions in the genome prior to and post induction of EBV's lytic phase by the BZLF1 protein. Two versions of BZLF1 were employed: full-length and activation-domain (AD)-truncated BZLF1 non-induced and induced for 15 h. The experim...
ORGANISM(S): Homo sapiens 
2019-04-01 | E-MTAB-7789 | biostudies-arrayexpress
TranscriptomicsChIP-seq against BZLF1 in Raji cells (EBV, Human Herpesvirus 4, GenBank accession KF717093).
ChIP-seq experiments with the antibody BZ1 directed against the EBV protein BZLF1 prior to and 15 hours post induction with doxycycline (100 ng/ml). The doxycycline regulated conditional BZLF1 allele is encoded by an inducible plasmid stably introduced into Raji cells. Experiments were performed as ...
ORGANISM(S): Homo sapiens 
2019-04-01 | E-MTAB-7788 | biostudies-arrayexpress
TranscriptomicsChIP-seq against the CTCF protein in Raji cells with inducible BZLF1 protein (KF717093.1) prior to and post induction of BZLF1.
Peaks were identified in both the human and viral (EBV (Human Herpesvirus 4; GenBank accession KF717093.1) genome.
ORGANISM(S): Homo sapiens 
2019-04-01 | E-MTAB-7787 | biostudies-arrayexpress
TranscriptomicsCHD1 remodelers space nucleosomes in vitro and link regular arrays to 5M-bM-^@M-^Y ends of genes in S. Pombe
Nucleosome positioning governs access to eukaryotic genomes. Many genes show a stereotypic organisation at their 5M-bM-^@M-^Y end: a nucleosome free region just upstream of the transcription start site (TSS) followed by a regular nucleosomal array over the coding region. The determinants for this pe...
ORGANISM(S): Schizosaccharomyces pombe 
2012-10-11 | E-GEOD-41024 | biostudies-arrayexpress
GenomicsStepwise reconstitution of genomic nucleosome organization with pure proteins
Nucleosome arrays begin at nucleosome-free promoter regions (NFRs) and regulate gene expression. Reconstituting such organization throughout a genome with purified proteins is a critical challenge in establishing biochemical mechanisms for chromosome assembly. Here we establish a four-step hierarchi...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-14 | E-GEOD-72106 | biostudies-arrayexpress
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