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TranscriptomicsReplication-coupled passive DNA demethylation for the erasure of genome imprints in mice
Genome-wide DNA demethylation, including the erasure of genome imprints, in primordial germ cells (PGCs), is critical as a first step for creating the totipotent epigenome in the germ line. Here, we provide evidence that contrary to the prevailing model involving active DNA demethylation, imprint er...
ORGANISM(S): Mus musculus 
2013-03-04 | E-GEOD-40412 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse germ cells with conditional Nanog knockdown.
Single Cell Microarray
E10.5 genital ridges (TM at E7.5) incubated in 0.5mM EDTA/ PBS for 20 minutes at 37ï¾°C were transferred to 2% BSA/ PBS. PGCs were collected from the genital ridges pierced with fine glass needles. Released PGCs were identified by their morphological characteristics and tr...
ORGANISM(S): Mus musculus 
2009-11-16 | E-MEXP-2411 | biostudies-arrayexpress
TranscriptomicsOffspring from oocytes derived from in vitro primordial germ cell-like cells in mice
Reconstitution of female germ-cell development in vitro is a key challenge in reproductive biology and medicine. We show here that female (XX) embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in mice are induced into primordial germ cell-like cells (PGCLCs), which, when aggrega...
ORGANISM(S): Mus musculus 
2012-11-05 | E-GEOD-40716 | biostudies-arrayexpress
TranscriptomicsInduction of the mouse germ cell fate by transcription factors in vitro [exp1]
The germ cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have established a culture system that recapitulates the mouse germ-cell specification pathway: Using cytokines, embryonic stem cells (ESCs)/induced plu...
ORGANISM(S): Mus musculus 
2013-08-04 | E-GEOD-43775 | biostudies-arrayexpress
TranscriptomicsCell-to-cell gene expression variability followed by reinforcement progressively segregates lineages in the early mouse embryo
Mammalian embryonic development begins with the specification and segregation of the two extra-embryonic lineages, trophectoderm and primitive endoderm, from the pluripotent embryonic lineage, the epiblast. To establish a map of epiblast (EPI) versus primitive endoderm (PrE) lineage segregation whic...
ORGANISM(S): Mus musculus 
2013-11-26 | E-MTAB-1681 | biostudies-arrayexpress
TranscriptomicsInduction of the mouse germ cell fate by transcription factors in vitro [exp2]
The germ cell lineage ensures the continuity of life through the generation of male and female gametes, which unite to form a totipotent zygote. We have established a culture system that recapitulates the mouse germ-cell specification pathway: Using cytokines, embryonic stem cells (ESCs)/induced plu...
ORGANISM(S): Mus musculus 
2013-08-04 | E-GEOD-46854 | biostudies-arrayexpress
GenomicsPRDM14 Ensures Naïve Pluripotency through Dual Regulation of Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells
Mouse embryonic stem cells (mESCs) fluctuate between a naïve inner cell mass (ICM)-like state and a primed epiblast-like state of pluripotency in serum, but are harnessed exclusively in a distinctive, naïve state of pluripotency that more faithfully captures the ICM state (the ground state) with i...
ORGANISM(S): Mus musculus 
2013-01-18 | E-GEOD-42616 | biostudies-arrayexpress
TranscriptomicsPRDM14 Ensures a Naïve Pluripotency by a Dual Mechanism Involving Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells
Mouse embryonic stem cells (mESCs) fluctuate between a naïve inner cell mass (ICM)-like state and a primed epiblast-like state of pluripotency in serum, but are harnessed exclusively in a distinctive, apparently more naïve state of pluripotency (the ground state) with inhibitors for mitogen-activate...
ORGANISM(S): Mus musculus 
2013-01-18 | E-GEOD-42580 | biostudies-arrayexpress
GenomicsA mesodermal factor, T (Brachyury), specifies mouse germ cell fate by directly activating germline determinants
Germ cells ensure reproduction and heredity in metazoans. Primordial germ cells (PGCs) in mice are induced in pluripotent epiblasts by BMP4 and WNT3, yet their mechanism of action remains unclear. Here, using in vitro PGC specification system, we show that WNT3 induces many transcription factors a...
ORGANISM(S): Mus musculus 
2013-12-11 | E-GEOD-50394 | biostudies-arrayexpress
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