Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to test the effects of OTUD6B-knockdown on the mRNA of KYSE30 cells. Methods: KYSE30 cells were transfected with negative control or siO...
Purpose: To fully realize the potential molecular mechanism that LHX2 promotes ESCC progression Methods: Total RNA of LHX2-knockdown KYSE30/KYSE510 and control cells was extracted with TRIzol Reagent. RNA libraries were constructed using an Illumina TruSeq RNA Sample Preparation kit according to the...
Purpose:RNA sequencing explored the possible altered gene transcriptome and signal pathways between ACh-stimulated KYSE30 cells and the respective control cells. Methods:RNA sequencing explored the ACh-stimulated KYSE30 cells and the respective control cells.