Sort   by:  
 Page size 
GenomicsDirected evolution improves the catalytic efficiency of APEX2-mediated proximity-dependent RNA labeling
Directed evolution improves the catalytic efficiency of APEX2-mediated proximity-dependent RNA labeling
PRJNA1090511 | ENA
ProteomicsTMT labeling for the masses: A robust and cost-efficient, in-solution labeling strategy
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quan...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-01-14 | PXD012703 | Pride
Stable isotope labeling Tandem mass tags Nhs ester chemistry Labeling efficiency 
ProteomicsSILAC labeling efficiency for c-di-GMP and CobB
Description To determine whether c-di-GMP could affect CobB-dependent deacetylation in a global setting, we applied Stable Isotope Labeling with Amino acids in Cell culture (SILAC) coupled with MS to quantitatively compare the levels of protein acetylation in WT, ΔcobB and ΔdgcZ cells. Before that, ...
ORGANISM(S): Escherichia coli 
2019-06-24 | PXD014344 | Pride
Silac c-di-gmp 
TranscriptomicsTranscription profiling of human peripheral blood mononuclear cells to compare one-cycle target labeling assays from Affymetrix, Enzo Life Sciences and Invitrogen Life Technologies
Comparison of One-Cycle Target Labeling Assay from Affymetrix, Enzo Life Sciences and Invitrogen Life Technologies
ORGANISM(S): Homo sapiens 
2006-11-30 | E-MEXP-884 | biostudies-arrayexpress
TranscriptomicsGene expression profiling on FePro labeled bone marrow stromal cells
Superparamagnetic iron oxide nanoparticles (SPION) are increasingly used to label human bone marrow stromal cells (BMSCs, also called mesenchymal stem cells) to monitor their fate by in vivo MRI and histology after Prussian blue (PB) staining. SPION-labeling appears to be safe as assessed by in vitr...
ORGANISM(S): Homo sapiens 
2011-01-24 | E-GEOD-20431 | biostudies-arrayexpress
TranscriptomicsDirected evolution improves the catalytic efficiency of APEX2-mediated proximity-dependent RNA labeling
Engineered ascorbate peroxidase APEX2 has seen widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin-aniline (Btn-An) remains suboptimal. To overcome this limitation, we performed yeast surface display ba...
ORGANISM(S): Homo sapiens 
2026-03-26 | GSE262170 | GEO
ProteomicsBioorthogonal Photocatalytic Proximity Labeling in Primary Living Samples
In situ profiling of subcellular proteomic networks in primary and living systems, such as primary cells from native tissues or clinic samples, is crucial for the understanding of life processes and diseases, yet challenging for the current proximity labeling methods (e.g., BioID, APEX) due to their...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-05-24 | PXD045791 | Pride
Proximity labeling Mitochondria Bioorthogonal chemistry Photocatalysis 
ProteomicsImproved SILAC Method for Double Labeling of Bacterial Proteome
Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quanti...
ORGANISM(S): Staphylococcus aureus 
2022-03-02 | PXD011221 | Pride
Proteomics Double labeling Bacteria Silac 
ProteomicsImproved SILAC Method for Double Labeling of Bacterial Proteome
Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quanti...
ORGANISM(S): Escherichia coli Staphylococcus aureus 
2022-02-28 | PXD011244 | Pride
Proteomics Double labeling Bacteria Silac 
UnknownBidirectional crusher gradient method to estimate the labeling efficiency of pseudo-continuous arterial spin labeling MRI in mice.
Not available
S-EPMC12681292 | biostudies-literature
Sort   by:  
 Page size