SILAC labeling efficiency for c-di-GMP and CobB

ABSTRACT: Description To determine whether c-di-GMP could affect CobB-dependent deacetylation in a global setting, we applied Stable Isotope Labeling with Amino acids in Cell culture (SILAC) coupled with MS to quantitatively compare the levels of protein acetylation in WT, ΔcobB and ΔdgcZ cells. Before that, we determined the labeling efficiency for these samples.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Zhaowei Xu  

LAB HEAD: Zhaowei Xu

PROVIDER: PXD014344 | Pride | 2019-06-24

REPOSITORIES: Pride