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TranscriptomicsTranslation profiling of Schizosaccharomyces pombe size separated polysome fractions provides data on average numbers of associated ribosomes for most transcripts
To obtain translational profiles for all mRNAs, polysome preparations are separated according to their size using a sucrose gradient and the mRNAs in each fraction are identified and quantified with DNA microarrays. Starting with exponentially growing cells, we analyzed 12 polysome fractions using D...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-222 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation to measure RNA polymerase II occupancy in fission yeast
The relative amount of RNA polymerase II (Pol II) associated with a given ORF provides an estimate for transcriptional efficiency. We therefore established a systematic approach to measure Pol II occupancy using chromatin immunoprecipitation followed by analysis on microarrays
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-219 | biostudies-arrayexpress
TranscriptomicsPoly(A) tail length profiling of mRNAs from fission yeast
To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarr...
ORGANISM(S): Schizosaccharomyces pombe 
2007-05-16 | E-TABM-217 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of logarithmically growing fission yeast
To estimate mRNA steady-state levels we used RNA extracted from logarithmically growing fisson yeast cells on Affymetrix Yeast 2.0 Genechip arrays. The signal intensities from two independent biological repeats were averaged, resulting in measurements for 4818 out of 4962 nuclear protein-coding gen...
ORGANISM(S): Schizosaccharomyces pombe 
2007-03-22 | E-TABM-220 | biostudies-arrayexpress
TranscriptomicsIMR90 hTERT
Transcription profiling from young and pre-senescent IMR90 cells, transfected either with hTERT (pBabe-puro-hTERT) or vector control (pBabe-puro). Population doubling values are contained in Characteristics[generation] column.
ORGANISM(S): Homo sapiens 
2014-06-17 | E-MTAB-2087 | biostudies-arrayexpress
TranscriptomicsIMR90 DDR
Transcription profiling of IMR90 fibroblasts after ionizing radiation. Cells were harvested 30 minutes (acute response) or 5 days (cell cycle arrested) after being irradiated with 5Gy of ionizing radiation.
ORGANISM(S): Homo sapiens 
2014-06-17 | E-MTAB-2088 | biostudies-arrayexpress
TranscriptomicsIMR90 Senescence
Transcription profiling from human primary fibroblasts as they progress into senescence. Samples were taken early population doubling onwards until cells reached replicative senescence and stopped dividing. Population doubling values are contained in Characteristics[generation] column.
ORGANISM(S): Homo sapiens 
2014-06-17 | E-MTAB-2086 | biostudies-arrayexpress
TranscriptomicsIMR90 Quiescence
Transcription profiling from human primary fibroblasts grown either in the presence of serum (15% FBS) or arrested after serum starvation (0% FBS).
ORGANISM(S): Homo sapiens 
2014-06-17 | E-MTAB-2105 | biostudies-arrayexpress
TranscriptomicsASF1
Transcription profiling by array of HeLa cells after the knock-down of ASF1 (ASF1a & ASF1b) by siRNA compared to non-targeting control siRNA
ORGANISM(S): Homo sapiens 
2014-01-14 | E-MTAB-1909 | biostudies-arrayexpress
UnknownRandomized Open-Label Phase II Trial of Apitolisib (GDC-0980), a Novel Inhibitor of the PI3K/Mammalian Target of Rapamycin Pathway, Versus Everolimus in Patients With Metastatic Renal Cell Carcinoma.
Not available
S-EPMC5569691 | biostudies-literature
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