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TranscriptomicsTranscriptomic sequencing of the murine testis during the first wave of spermatogenesis
Our experimental design includes samples at different time points during the first wave of spermatogenesis. Each time point corresponds to specific cell content in the testis. First time point is post natal day (PND) 7, when only somatic cells and spermatogonia are present in the testis. Thereafter ...
ORGANISM(S): Mus musculus 
2013-04-29 | E-GEOD-39970 | biostudies-arrayexpress
TranscriptomicsGene expression in Drosophila montana
We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during ...
ORGANISM(S): Drosophila montana 
2010-05-06 | E-GEOD-18475 | biostudies-arrayexpress
TranscriptomicsAnalysis of HSF2 target genes using a global promoter ChIP-chip strategy on mouse wild-type testis
Mapping of in vivo targets for HSF2 by using mammalian tissue material in a global ChIP-chip promoter screen. Chromatin was isolated from three wild-type (WT) mouse testes, crosslinked and sonicated into fragments of 100-500 bp, and the quality of the DNA was controlled before the immunoprecipitatio...
ORGANISM(S): Mus musculus 
2008-08-05 | E-GEOD-9289 | biostudies-arrayexpress
TranscriptomicsCharacterization of gene expression involved in pig reproduction
Purpose: Recent development in high-throughput sequencing techniques (RNA-seq) has enabled large scale analysis of genetic variations and gene expression in different tissues and species, but gene expression patterns and genetic variations in livestock have not yet been well characterized. In this s...
ORGANISM(S): Sus scrofa 
2015-08-06 | E-GEOD-59149 | biostudies-arrayexpress
TranscriptomicsComparison of hypoxia (4 % O2) cultured human embryonic stem cells (hESCs) to normoxia (21 % O2) cultured
We compared the transcriptome at gene expression level in hypoxic and normoxic conditions. The transcriptome of cells cultured at three different timepoints, 2h, 24 h and 7 days with three different cell lines in normoxia and hypoxia was compared 18 samples total, 3 replicates in each conditions, 2h...
ORGANISM(S): Homo sapiens 
2014-01-02 | E-GEOD-35819 | biostudies-arrayexpress
TranscriptomicsEffect of Tet1-knockdown on gene expression in mouse ES cells cultured in ES and TS cell culture conditions
TET-family enzymes convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in DNA. Tet1 and Tet2 are Oct4-regulated enzymes that together sustain 5hmC in mouse embryonic stem (ES) cells. ES cells depleted of Tet1 by RNAi show diminished expression of the Nodal antagonist Lefty1, and displ...
ORGANISM(S): Mus musculus 
2011-02-07 | E-GEOD-26900 | biostudies-arrayexpress
TranscriptomicsHSF1 regulates transcription of sex-chromosomal multicopy genes during post-meiotic repression
Mapping of in vivo targets for HSF1 by using mammalian tissue material in a global ChIP-chip promoter screen. Chromatin was isolated from three wild-type (WT) mouse testes, crosslinked and sonicated into fragments of 100-500 bp, and the quality of the DNA was controlled before the immunoprecipitatio...
ORGANISM(S): Mus musculus 
2010-08-31 | E-GEOD-22492 | biostudies-arrayexpress
TranscriptomicsSOLiD sequencing of Noccaea (Thlaspi) caerulescens root transcriptomes from three contrasting ecotypes
Root transcriptomes were sequenced using SOLiD 4 chemistry. The three accessions LC, GA and MP differed significantly in the expression of genes that contribute to metal ion transport and detoxification, stress tolerance and secondary metabolite biosynthesis. These data provide comprehensive genome-...
ORGANISM(S): Noccaea caerulescens 
2014-02-28 | E-GEOD-35900 | biostudies-arrayexpress
TranscriptomicsGene analysis of the neonatal rat lung exposed to STZ-induced maternal diabetes
Maternal diabetes is associated with a wide range of fetal and neonatal adverse effects including pulmonary disturbances. To investigate the effects of maternal diabetes on neonatal lung gene expression profile, we performed microarray analysis on the lungs of 14-day-old rats born to diabetic dam. ...
ORGANISM(S): Rattus norvegicus 
2009-01-07 | E-GEOD-14307 | biostudies-arrayexpress
TranscriptomicsWhole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen.
We have utilized the raw sequence data from our earlier investigation of the lichen transcriptome to design a custom DNA microarray for C. rangiferina in order to study the transcripts expressed in lichen thallus during dehydration and rehydration. The aim of this study was to identify the genes mos...
ORGANISM(S): Cladonia rangiferina 
2013-11-25 | E-GEOD-47624 | biostudies-arrayexpress
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