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TranscriptomicsRNA-seq of murine spleen B-cells after pentanoate treatment compared to untreated control
We tested the influence of pentanoate on B cells by treatment of CpG-stimulated B cells in vitro. We isolated naive B cells from FIR/TIGER mice (IL-10 (GFP) - Foxp3 (RFP) - Reporter mice) from spleen. Cells were cultured in presence of 5 µg/ml CpG 2395. 24h after CpG-stimulation, B cells were treate...
ORGANISM(S): Mus musculus 
2019-01-15 | E-MTAB-6115 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Drosophila melanogaster testis with or without tbrd-1 knock out or tplus3a/b knock out
Many protein complexes are involved in gene regulation during Drosophila spermatogenesis. tplus3a and tplus3b code for Plus3 domain proteins and are enriched in spermatocytes. Male flies ∆tplus3a/b carrying deletions of both genes in trans to deficiency Df(2L)BSC151 show severely reduced fertility. ...
ORGANISM(S): Drosophila melanogaster 
2019-11-04 | E-MTAB-7013 | biostudies-arrayexpress
TranscriptomicsRNA-seq of murine CD4 T cells after pentanoate treatment compared to untreated control
To analyse the influence of pentanoate on the differentiation of naive CD4 T cells under Th17 inducing conditions, murine CD4 T cells were isolated from spleen and lymphnodes of FIR/TIGER mice (IL-10 (GFP) - Foxp3 (RFP) - Reporter mice). The T cells were kept for 3 days under Th17 inducing condition...
ORGANISM(S): Mus musculus 
2019-01-15 | E-MTAB-6114 | biostudies-arrayexpress
GenomicsChIP-Seq of SMARCAD1 and KAP1 in male mouse embryonic stem cells (E14)
The ATP dependent chromatin remodeler SMARCAD1 and the co-repressor KAP1 (Trim28/Tif1 beta) interact directly. We wanted to understand the interplay between these two proteins in the context of chromatin; in particular at repetitive sequences. Therefore, we carried out an investigation in E14 ES cel...
ORGANISM(S): Mus musculus 
2019-02-17 | E-MTAB-7011 | biostudies-arrayexpress
GenomicsChIP-seq of FLAG-tagged SMARCAD1 in female mouse embryonic stem cells (PGK12.1 XX)
To understand the role of the SWI/SNF-like ATP-dependent chromatin remodeller SMARCAD1 in pluripotent murine embryonic stem (ES) cells we determined the genome wide binding of triple FLAG tagged SMARCAD1 in PGK12.1 XX ES cells. ChIP was carried out using an antibody against the FLAG-epitope (F1804, ...
ORGANISM(S): Mus musculus 
2019-02-17 | E-MTAB-7014 | biostudies-arrayexpress
GenomicsChIP-Seq of chromatin remodeler SMARCAD1 and H3K9me3 in female mouse embryonic stem cells (PGK12.1 XX)
Investigation of the co-occurance of the ATP-dependent chromatin remodeler SMARCAD1 and the histone modification H3K9me3 in the genome of PGK12.1 XX ES cells. ChIP-seq of endogenous SMARCAD1 was carried out in control and SMARCAD1-knockdown ES cells using a double crosslinking procedure. Additionall...
ORGANISM(S): Mus musculus 
2019-02-17 | E-MTAB-7012 | biostudies-arrayexpress
GenomicsChipSeq of conditional knock-in mouse carrying the R178E (EE) mutation in exon 5 of the endogenous mouse Trp53 gene locus.
The mouse R178E (EE) mutation of the Trp53 is a p53 mutant protein with native conformation that lacks the ability to form tetramers and thus constitutes a mutant form of p53 that lacks DNA binding cooperativity. Here we want to assess DNA binding ability of the EE mutant in MEFs under untreated con...
ORGANISM(S): Mus musculus 
2019-09-03 | E-MTAB-6793 | biostudies-arrayexpress
TranscriptomicsResidual tumor suppressor activities of tumorigenic p53 mutants enhance survival under chemotherapy
p53 mutations are believed to correlate with poor patient survival, as mutations abolish the transcriptional function of wild-type p53 in the DNA damage response and mutant p53 gain-of-function activities enhance drug resistance. Moreover, p53 also exhibits non-transcriptional apoptotic activity wit...
ORGANISM(S): Mus musculus 
2019-09-03 | E-MTAB-6774 | biostudies-arrayexpress
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