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We show that lanthanum concentration and lanthanide species significantly affect gene expression under methylotrophic growth conditions. Using incubations with either lanthanum (La), neodymium (Nd), or a lanthanide cocktail, differential gene expression analysis revealed that up tp 41% of the encode...
ORGANISM(S): Beijerinckiaceae bacterium RH AL1 
Lanthanides were recently discovered as metals required in the active site of certain methanol dehydrogenases. Since then, the characterization of the lanthanome, i.e. all proteins involved in sensing, uptake, and utilization of lanthanides, has become an active field of research. Initial exploratio...
ORGANISM(S): Methylobacillus flagellatus KT 
2023-01-31 | PXD032828 | Pride
We investigated lanthanide (Ln) utilization in Beijerinckiaceae bacterium RH AL1, which requires light Lns (La–Nd) for methanol oxidation. The strain was grown with methanol as the carbon and energy source and with Ln sources including natural minerals (apatite, bastnäsite, gadolinite, loparite, mon...
ORGANISM(S): Beijerinckiaceae bacterium 
Several of the metabolic enzymes in methanotrophic bacteria rely on metals for both their expression and their catalysis. The MxaFI methanol dehydrogenase enzyme complex uses calcium as a cofactor to oxidize methanol, while the alternative methanol dehydrogenase XoxF uses lanthanide metals such as l...
ORGANISM(S): Methylotuvimicrobium buryatense 
2019-05-21 | GSE125909 | GEO
Discovery and characterization of the first known biological lanthanide chelator
Gene expression changes in response to lanthanum concentration and lanthanide species in Beijerinckiaceae bacterium RH AL1
The effect of lanthanum concentration and the effect of different lanthanide elements on gene expression and growth was tested in P. alloputida KT2440. Gene expression analysis was based on high-throughput RNAseq starting from rRNA-depleted samples, followed by determining differentially expressed g...
ORGANISM(S): Pseudomonas putida 
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