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TranscriptomicsBiochemical Identification of Targets of Individual MicroRNAs in Mouse Embryonic Stem Cells
Mouse Embryonic Stem (ES) cells express a unique set of microRNAs (miRNAs), the miR-290-295 cluster. To elucidate the role of these miRNAs and how they integrate into the ES cell regulatory network requires identification of their direct regulatory targets. The difficulty, however, arises from the l...
ORGANISM(S): Mus musculus 
2010-02-27 | E-GEOD-20199 | biostudies-arrayexpress
TranscriptomicsGlobal Deterministic and Stochastic Allelic Specific Gene Expression in Single Blastomeres of Mouse Early Embryos
Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a singl...
ORGANISM(S): Mus musculus 
2010-12-31 | E-GEOD-22182 | biostudies-arrayexpress
TranscriptomicsTracing the derivation of embryonic stem cells from the inner cell mass by single cell RNA-seq analysis
Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a singl...
ORGANISM(S): Mus musculus 
2013-06-11 | E-GEOD-20187 | biostudies-arrayexpress
GenomicsBLIMP1 ChIPseq
As a part of a modelling experiment for transcriptional control of mouse primordial germ cell specification, the transcription factor BLIMP1 was transiently expressed in the mouse p19 embryonal carcinoma cell line and its genome wide binding sites were defined using ChIPseq.
ORGANISM(S): Mus musculus 
2013-07-17 | E-MTAB-1599 | biostudies-arrayexpress
GenomicsAP2gamma ChIPseq
As a part of a modelling experiment for transcriptional control of mouse primordial germ cell specification, the transcription factor AP2gamma was stably expressed in the mouse p19 embryonal carcinoma cell line and its genome wide binding sites were defined using ChIPseq.
ORGANISM(S): Mus musculus 
2013-07-17 | E-MTAB-1600 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of brain tumor cells to assess endogenous mRNA fluctuations detects a microRNA signal in an in vivo expression set of mRNAs.
Background: microRNAs (miRNAs) are approximately 21 nucleotide non-coding transcripts capable of regulating gene expression. The most widely studied mechanism of regulation involves binding of the miRNA to a target mRNA, usually in its 3’ untranslated region (UTR). As a result, translation of t...
ORGANISM(S): Homo sapiens 
2009-04-25 | E-GEOD-8692 | biostudies-arrayexpress
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