We assessed alternative splicing in breast cancer through global profiling of transcriptomes of basal and luminal subtype cell lines using Affymetrix Human Junction Array.
DNA from 30 cultured ovarian cancer cell lines was extracted with Promega kits and hybridize to arrays. Arrays comprised of 1860 BACs selected to include genes known to be involved in cancer pathogenesis and 400 BACs selected to tile across 13 Mbp at 3q26, 15 Mbp at 8q24, and 30 Mbp at 20q centered ...
RNA from 30 ovarian cancer lines was extracted when cells were growth to 70-80% confluency. Total RNA was extraced with Qiagen Rneasy columns and evaluated for quality by Bioanalyzer.
