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The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 ...
ORGANISM(S): Rattus norvegicus 
Lim Mineralization Protein, splice variant-3 (LMP3) is known to induce osteoblast differentiation as a result of active modulation of selected molecules involved in the osteogenic cascade. LMP3 seems to act as an upstream modulator of osteogenic differentiation, although the complete network of mole...
ORGANISM(S): Homo sapiens 
The human neuroblastoma cell line Lan5 cells were cultured in complete DMEM till 80% confluence, then placed at 60000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 h...
ORGANISM(S): Homo sapiens 
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