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ProteomicsFunctional plasticity of HCO3- uptake and CO2 fixation in Cupriavidus necator H16
Uptake and fixation of CO2 are central to strategies for CO2-based biomanufacturing. Cupriavidus necator H16 has emerged as a promising industrial host for this purpose. Despite its prominence, the ability to engineer C. necator inorganic carbon uptake and fixation is underexplored. Here, we test th...
ORGANISM(S): Cupriavidus necator 
2026-01-23 | PXD051976 | Pride
Gas fermentation Dab Rubisco Carbonic anhydrase C1 metabolism Co2 biomanufacturing 
ProteomicsDevelopment of Corynebacterium glutamicum as a monoterpene production platform
Monoterpenes are commonly known for their role in the flavors and fragrances industry and are also gaining attention for other uses like insect repellant and as potential renewable fuels for aviation. Corynebacterium glutamicum, a Generally Recognized as Safe microbe, has been a choice organism in i...
ORGANISM(S): Corynebacterium glutamicum 
2024-02-28 | PXD044193 | Pride
Monoterpene biosynthetic production  geraniol  citronellol  eucalyptol  linalool  citral  geranic acid  corynebacterium glutamicum 
ProteomicsModular bottom-up proteomic sample preparation for high-throughput applications
A collection of digital automated proteomic sample preparation protocols detail three optimized step-by-step methods to: (A) lyse Gram-negative bacteria and fungal cells; (B) quantify the amount of protein extracted; and (C) normalize the amount of protein and set up tryptic digestion. These protoco...
ORGANISM(S): Rhodotorula toruloides Pseudomonas putida 
2022-02-14 | PXD029122 | Pride
High-throughput Proteomic sample preparation Automation 
ProteomicsDiscovery and Characterization of a Novel Carbohydrate-Active Enzyme from the Anaerobic Fungus Piromyces finnis
Dwelling in the gut of herbivores, anaerobic gut fungi (AGF) have evolved several strategies to efficiently degrade unpretreated biomass. Their genomes encode a diverse array of carbohydrate-active enzymes (CAZymes), yet exceedingly few of these enzymes have been validated. Here, we developed a pred...
ORGANISM(S): Escherichia coli 
2025-07-31 | PXD061558 | Pride
Cazymes Jbei Proteomics High-throughput screen Heterologous expression 
ProteomicsA rapid methods development workflow for high-throughput quantitative proteomic applications
we developed a workflow using data acquired from discovery proteomic experiments, retention time prediction, and standard-flow chromatography to rapidly develop targeted proteomic assays
ORGANISM(S): Escherichia coli 
2019-06-27 | PXD011212 | Pride
Workflow Proteomics High-throughput 
ProteomicsThe global response of Pseudomonas putida to a complex lignolysate
In this work, we developed processes using ionic liquids to obtain size-defined soluble aromatic-rich streams from sorghum biomass to assess their biocompatibility with P. putida. Growth was unexpectedly higher than that observed on a main aromatic compound. Subsequently, proteomics and metabolomics...
ORGANISM(S): Pseudomonas putida KT2440 
2020-04-08 | PXD014285 | Pride
Pseudomonas putida Proteomics Lignin valorization 
ProteomicsEngineering an Extremely Hybrid PKS for Adipic Acid Production
Polyketide synthases (PKSs) are modular enzymes with exceptional potential as biocatalysts for producing non-native compounds. Here, we report the first PKS-based pathway for adipic acid (AA), an industrial monomer for nylon production, by engineering one of the most extensively hybridized PKS syste...
ORGANISM(S): Pseudomonas putida KT2440 
2026-09-07 | PXD069956 | Pride
Hybrid pks Polyketide synthase Adipic acid P. putida Jbei E. coli 
UnknownCryo-EM of the human insulin receptor ectodomain in complex with an insulin analog with truncated B chain and enlongated A chain
Not available
EMPIAR-10736 | biostudies-other
ProteomicsAdaptive lab evolution and omics characterization enable tolerance engineering of Pseudomonas putida KT2440 to produce the jet-fuel precursor isoprenol
Pseudomonas putida KT2440 (KT2440) has been established as an industrially relevant chassis for the production of the jetfuel precursor isoprenol. However, this strain assimilated isoprenol as sole carbon source and also showed impaired growth at isoprenol concentrations > 2 g/L which is a significa...
ORGANISM(S): Pseudomonas putida KT2440 
2025-05-28 | PXD054609 | Pride
Pseudomonas putida Sustainable aviation fuel Isoprenol Adaptive laboratory evolution Tolerance engineering 
ProteomicsSimultaneous Optimization of Multi-lignocellulosic-sugar Catabolism via Systematic Laboratory Evolution in Dynamic Conditions
The development of microbial bioproduction is challenged by the complexity and variability of industrial feedstocks. This study aimed to systematically and simultaneously optimize catabolism of multiple sugars present in common renewable lignocellulose by adaptive laboratory evolution (ALE) using dy...
ORGANISM(S): Pseudomonas putida KT2440 
2026-06-29 | PXD055215 | Pride
Pseudomonas putida Adaptive laboratory evolution (ale) Proteomics Lignocellulose 
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