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TranscriptomicsCharacterization of small RNA-generating regions targeted by RNA polymerase V in Arabidopsis
In plants, heterochromatin is maintained by a small RNA-based gene silencing mechanism known as RNA-directed DNA methylation (RdDM). RdDM requires the non-redundant functions of two plant-specific DNA-dependent RNA polymerases (Pol) Pol IV and Pol V. Pol IV plays a major role in siRNA biogenesis, w...
ORGANISM(S): Arabidopsis thaliana 
2012-06-11 | E-GEOD-36424 | biostudies-arrayexpress
TranscriptomicsStructural Variant of a Tandem Repeat-containing Silencer Locus Reveals Distinct Pol II and Pol IV-dependent Pathways of siRNA Biogenesis
We used a two-component transgene system to study the RNA-dependent DNA methylation (RdDM) and transcriptional gene silencing (TGS) in Arabidopsis. By profiling the small RNA population in  mutants defected in RdDM or RNA polymerase II-transcribed trigger for generating silencing siRNA, we investiga...
ORGANISM(S): Arabidopsis thaliana 
2014-08-05 | E-GEOD-47852 | biostudies-arrayexpress
TranscriptomicsThe dicerlike1 homologue, fuzzy tassel, is required for the regulation of meristem determinacy in the inflorescence and vegetative growth in maize
We have characterized a maize mutant, fuzzy tassel (fzt) with striking inflorescence and pleiotropic defects. fzt contains a mutation in the maize dicer-like1 homolog, a key enzyme required for microRNA (miRNA) biogenesis. By profiling the small RNA population in fzt mutants, we investigated the imp...
ORGANISM(S): Zea mays 
2015-02-20 | E-GEOD-52879 | biostudies-arrayexpress
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