Characterization of small RNA-generating regions targeted by RNA polymerase V in Arabidopsis

ABSTRACT: In plants, heterochromatin is maintained by a small RNA-based gene silencing mechanism known as RNA-directed DNA methylation (RdDM). RdDM requires the non-redundant functions of two plant-specific DNA-dependent RNA polymerases (Pol) Pol IV and Pol V. Pol IV plays a major role in siRNA biogenesis, while Pol V may recruit DNA methylation machinery to target endogenous loci for silencing. Although small RNA-generating regions which are dependent on both Pol IV and Pol V have been identified previously, the genomic loci targeted Pol V for siRNA accumulation and silencing have not been described extensively. To characterize the Pol V-dependent, heterochromatic siRNA-generating regions in the Arabidopsis genome, we deeply sequenced the small RNA populations of wild-type and Pol V mutant plants.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Blake Meyers 

PROVIDER: E-GEOD-36424 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress