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ProteomicsAc-LysargiNase Helps Genome Reannotation of Mycolicibacterium smegmatis MC2 155
To achieve deep coverage of M. smegmatis proteome, 240 μg proteins were reduced with 5 mM of dithiothreitol (DTT), alkylated with 20 mM of iodoacetamide (IAA). The alkylated proteins were separated by a 10% SDS-PAGE for 8 cm, and stained with Coomassie Blue G250. Gel lane was excised into 13 fractio...
ORGANISM(S): Mycolicibacterium Smegmatis Mc2 155 
2022-09-29 | PXD037080 |
ProteomicsIdentification of GSDME-interacting proteins in HK-2 cells by immunoprecipitation-coupled LC-MS/MS analysis
This project aims to identify GSDME-associated proteins in human renal tubular epithelial (HK-2) cells using immunoprecipitation followed by LC-MS/MS. HK-2 cells overexpressing Flag-tagged GSDME were lysed, and protein complexes were enriched using anti-Flag antibody and Protein A/G magnetic beads. ...
ORGANISM(S): Homo Sapiens 
2025-04-12 | PXD062902 |
ProteomicsA proximity labeling map of SARS-CoV-2 and human
The global epidemic caused by the coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in the infection of over 200 million people. To extend the knowledge of interactions between SARS-CoV-2 and humans, we systematically investigate the interactome of 29 viral protei...
ORGANISM(S): Homo Sapiens 
2022-01-06 | PXD022086 |
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