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ProteomicsAutophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Tandem Affinity Purification coupled to LC-MS/MS of SFB-TLK1 133-208 and 133-208 Y149A F150A)
Tandem affinity purification of HEK 293T cells transiently expressing SFB-TLK1 133-208 or SFB-TLK1 133-208 Y149A F150A to identify if TLK1 PIP box mutants can still interact with PCNA.
ORGANISM(S): Homo sapiens (Human) 
2025-01-02 | PXD048215 | Pride
Tandem affinity purification Tousled-like kinases Lc-ms/ms 
ProteomicsAutophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Phospho-TMT analysis of TLK1)
PhosphoTMT experiment on HEK293T cells transiently transfected with either SFB-TLK1, SFB-TLK2, or siRNAs targeting TLK1, TLK2, or TLK1 and TLK2. Data represents 4 biological replicates.
ORGANISM(S): Homo sapiens (Human) 
2025-01-02 | PXD047966 | Pride
Phosphoproteomics Tandem affinity purification Tousled-like kinases Phospho-tmt 
ProteomicsAutophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Tandem Affinity Purification coupled to LC-MS/MS of SFB-TLK1 WT and TLK2 WT)
S-protein-2xFLAG-Streptavidin binding peptide (SFB)-tagged TLK1 or SFB-TLK2 were transfected into 4x 15 cm plates of HEK293T cells. Cells were harvested 24 hours after transfection using NETN buffer (150 mM NaCl, 0.5 mM EDTA, 20 mM Tris-HCl pH 8.0, 0.5% NP-40) supplemented with 2 µg/mL aprotinin (Th...
ORGANISM(S): Homo sapiens (Human) 
2025-01-02 | PXD047996 | Pride
Tandem affinity purification Tousled-like kinases Lc-ms/ms 
ProteomicsAutophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Tandem Affinity Purification coupled to LC-MS/MS of SFB-TLK1 WT, 1-240, and D607A)
Tandem affinity purification of SFB-Tagged TLK1 Wildtype, TLK1 1-240, and TLK1 D607A from transiently transfected HEK 293T cells to try to identify possible TLK1 interactions. Data represent technical duplicates.
ORGANISM(S): Homo sapiens (Human) 
2025-01-02 | PXD047964 | Pride
Tandem affinity purification Tousled-like kinases Lc-ms/ms 
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