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ProteomicsAnalysis of oncogenic KRAS impact on the phospho-proteome of PDAC cells.
To reveal the impact of mutant KRAS on the phospho-proteome of Pancreatic Ductal Adenocarcinoma (PDAC) cells, we carried out a quantitative phospho-proteomic analysis of tumour cells isolated from an inducible mouse model of PDAC (iKras PDAC) (Ying et al., 2012). In this model, oncogenic Kras (G12D)...
ORGANISM(S): Mus musculus (Mouse) 
2023-02-12 | PXD030893 | Pride
Kras  tmt  phospho-proteomics  pdac  erk 
ProteomicsHiRIEF LC-MS allows for in depth quantitative analysis of the phosphoproteome.
In this project we aimed at improving phosphoproteomics analysis by taking advantage of high resolution isoelectric focusing (HiRIEF) fractionation. We developed a workflow that employs titanium dioxide phospho-enrichment, followed by isobaric labeling with Tandem Mass Tags (TMT) and HiRIEF on a bro...
ORGANISM(S): Homo sapiens (Human) 
2017-07-07 | PXD005410 | Pride
Multiplex Cells Isoelectric point Tmt Phospho-peptides Phospho-sites Fractionation Quantification Networkin Cancer Phosphoproteomics Tyrosine Ipg Proteomics Hirief 10-plex Lc-ms Quantitative Mitosis Mitotic arrest Hela Kinase association Multiply phosphorylated Phosphorylation Pervanadate 
ProteomicsInhibition of growth factor signaling prevents SARS-CoV-2 replication
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Homo sapiens (Human) 
2020-05-15 | PXD018357 | Pride
Infection Sars-cov-2 Phospho Tmt Covid-19 
ProteomicsQuantitative Global and Phospho-proteomics analysis of rapamycin-treated human SU-DHL-4 cells by LC-MSMS
Global protein profiling and global phospho-protein profiling in human diffuse large B cell lymphoma cell line SU-DHL-4 treated with Rapamycin at two time points compared with DMSO control was performed by Poochon Scientific using the TMT-18plex labeling based quantitative proteomics method and liqu...
ORGANISM(S): Homo sapiens (Human) 
2025-09-03 | PXD067812 | Pride
Lc-msms Global protein profiling Rapamycin Su-dhl-4 Tmt multiplex Global phospho-protein profiling Dlbcl 
ProteomicsSystematic evaluation of GAPs and GEFs identifies ARHGAP45 as a targetable leukemia-specific RhoGAP dependency
TMT-based proteomics and phospho-proteomics to identify the potential ARHGAP45-regulated downstream effectors in human AML cells.
ORGANISM(S): Homo sapiens (Human) 
2025-07-28 | PXD054537 | Pride
Aml Proteomics Tmt Gap45-ko Phospho-proteomics 
ProteomicsPhospho-Proteome Analysis of ROCK1 Conditional Knock-Out (ROCK1cKO) Mouse B-Cells under various in vitro stimulation conditions by LC-MS/MS
To better delineate how ROCK1 controls the biochemical state of activated B cells, we next conducted a phospho-proteomic analysis. In vitro stimulated WT and CD23-Rock1 B cells were harvested, subjected to pS/pT TMT and total proteome TMT, and results analyzed by Proteome Discoverer.
ORGANISM(S): Mus musculus (Mouse) 
2025-05-07 | PXD059659 | Pride
Stimulation conditions Mouse primary b-cells Rock1cko Lc-ms/ms 
UnknownStreamlined Tandem Mass Tag (SL-TMT) Protocol: An Efficient Strategy for Quantitative (Phospho)proteome Profiling Using Tandem Mass Tag-Synchronous Precursor Selection-MS3.
Not available
S-EPMC5994137 | biostudies-literature
ProteomicsOptimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing
In large-scale quantitative mass spectrometry (MS)-based phosphoproteomics, isobaric labeling with tandem mass tags (TMTs) coupled with offline high-pH reversed-phase peptide chromatographic fractionation maximizes depth of coverage. To investigate to what extent limited sample amounts affects sensi...
ORGANISM(S): Homo sapiens (Human) 
2022-06-28 | PXD031277 | Pride
Tandem mass tags Phosphoproteomics Optimization High-ph fractionation Isobaric labeling 
ProteomicsPhospho- and total- proteomic analysis of FAK knockout in primary mouse lung endothelial cells
We assessed the impact of Endothelial Focal Adhesion kinase (ECFAK) knockout, using primary lung endothelial cells isolated (at P10-P14) from a FAK flox/flox mice crossed with mice expressing a tamoxifen-inducible Cre(iCreERT2) that is driven by endothelial cell (EC)-selective platelet-derived growt...
ORGANISM(S): Mus musculus (Mouse) 
2022-04-13 | PXD030824 | Pride
Fak  endothelial cells  quantitative proteomics  vegf  tmt 
ProteomicsAutophosphorylation of Tousled-like kinases (TLK1 and TLK2) regulates recruitment to damaged chromatin via PCNA interaction (Phospho-TMT analysis of TLK1)
PhosphoTMT experiment on HEK293T cells transiently transfected with either SFB-TLK1, SFB-TLK2, or siRNAs targeting TLK1, TLK2, or TLK1 and TLK2. Data represents 4 biological replicates.
ORGANISM(S): Homo sapiens (Human) 
2025-01-02 | PXD047966 | Pride
Phosphoproteomics Tandem affinity purification Tousled-like kinases Phospho-tmt 
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