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ProteomicsThermococcus kodakarensis proteins expressed at various temperatures reveals changes in RNA modifications
Thermococcus kodakarensis is a thermophilic archaea used in this study to determine how changes in growth temperature affect protein expression. This organism was chosen, in part, due to the simplified proteome and potential protein stability gained when analyzing proteins at room temperature. Two t...
ORGANISM(S): Thermococcus kodakarensis KOD1 
2020-02-17 | PXD014814 | Pride
Proteomics Thermococcus kodakarensis Theromphile Qexactive plus 
ProteomicsProbing the Sensitivity of the Lumos Mass Spectrometer by analyzing varying amounts of whole cell extracts
PROJECT DESCRIPTION The Orbitrap Fusion Lumos mass spectrometer has recently been shown to have better fragmentation and detection than its predecessors. Previous studies using a HeLa digest analyzed on the Lumos mass spectrometer have reported the number of peptides and proteins identified by rever...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2018-04-19 | MSV000082296 | MassIVE
ProteomicsMudPIT analysis of S. pombe proteins interacting with Pof8
To identify proteins interacting with Pof8, cell-free extracts of strains FP1547 harboring 3xFLAG epitope-tagged Pof8 and untagged control (FP1546) were diluted to 5 mg/ml in 8 ml of lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM sodium chloride, 0.1% (v/v) NP40 and 10% (v/v) glycerol) plus complete ED...
ORGANISM(S): Schizosaccharomyces Pombe (ncbitaxon:4896) 
2021-05-24 | MSV000087493 | MassIVE
ProteomicsFGF2 antiproliferative stimulus triggers proteomic and transcriptional changes associated with nucleolar disorganization in K-ras-driven mouse tumor cells (Chromatin MudPIT data)
Chromatin plays a crucial role in the intermediation between cell signaling and gene expression. The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has anti-proliferative and tumor-suppressive functio...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-10-11 | MSV000090511 | MassIVE
ProteomicsFGF2 antiproliferative stimulus triggers proteomic and transcriptional changes associated with nucleolar disorganization in K-ras-driven mouse tumor cells (Nucleoli TMT)
Chromatin plays a crucial role in the intermediation between cell signaling and gene expression. The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has anti-proliferative and tumor-suppressive functio...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-10-12 | MSV000090518 | MassIVE
ProteomicsFGF2 antiproliferative stimulus triggers proteomic and transcriptional changes associated with nucleolar disorganization in K-ras-driven mouse tumor cells (CLASP MudPIT data)
Chromatin plays a crucial role in the intermediation between cell signaling and gene expression. The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has anti-proliferative and tumor-suppressive functio...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-10-12 | MSV000090516 | MassIVE
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