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ProteomicsOrganization, genomic targeting and assembly of three distinct SWI/SNF chromatin remodeling complexes in Arabidopsis
To comprehensively define the potential plant SWI/SNF sub-complexes and their subunits organization, we started by performing immuno-purification followed by mass spectrometry analysis (IP-MS) using stable transgenic Arabidopsis lines that expressed a green fluorescent protein (GFP)-tagged ATPase su...
ORGANISM(S): Arabidopsis Thaliana 
PXD041567 |
ProteomicsBCL7A and BCL7B potentiate SWI/SNF-complex-mediated chromatin accessibility to regulate gene expression and vegetative phase transition in Arabidopsis
Arabidopsis thaliana, 14-day-old seedlings grow in 1/2 MS culture medium under short day condition, LC-MS. In this project, two different materials (One is in the wild type background, the other is in the mutant background.) were used to do CoIP experiments, and then mass spectrometry was carried ou...
ORGANISM(S): Arabidopsis Thaliana 
2024-01-03 | PXD045757 |
GenomicsGenome-wide profiling of trimethylated histone H3 lysine 27 (H3K27me3) in wide-type, brm, clf, and brm clf mutant seedlings
By using ChIP-seq, we found that loss of BRM activity in developing seedlings leads to ectopic and increased H3K27me3 deposition at several hundred genes, indicating the critical role of BRM in preventing the inappropriate deposition of this histone mark. Removal of CLF in brm mutant could partciall...
ORGANISM(S): Arabidopsis thaliana 
2015-03-09 | E-GEOD-53620 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of SWI/SNF chromatin remodeler BRAHMA and Histone H3 lysine demethyalse Relative of Early Flowering 6 (REF6) in Arabidopsis [RNA-Seq]
BRAHMA (BRM) is a conserved SWI/SNF-type chromatin remodeling ATPase implicated in many key nuclear events. Histone H3 Lysine 27 (H3K27) demethylases specifically remove the repressive histone mark, trimethylation of H3K27 (H3K27me3). Both proteins are thought to play active roles in regulating gene...
ORGANISM(S): Arabidopsis thaliana 
2016-03-28 | E-GEOD-72260 | biostudies-arrayexpress
GenomicsGenome-wide profiling of SWI/SNF chromatin remodeler BRAHMA and Histone H3 lysine demethyalse Relative of Early Flowering 6 (REF6) in Arabidopsis [ChIP-Seq]
BRAHMA (BRM) is a conserved SWI/SNF-type chromatin remodeling ATPase implicated in many key nuclear events. Histone H3 Lysine 27 (H3K27) demethylases specifically remove the repressive histone mark, trimethylation of H3K27 (H3K27me3). Both proteins are thought to play active roles in regulating gene...
ORGANISM(S): Arabidopsis thaliana 
2016-03-28 | E-GEOD-72735 | biostudies-arrayexpress
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