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Proteomics,Multiomicsembryo_proteome
We collected over 8000 mouse embryos of each developmental stage (zygote, 2-cell, 4-cell, 8-cell, morula, blastocyst) and performed tandem mass tag (TMT)-based quantitative mass spectrometry and identified nearly 5000 proteins for each stage. In-depth analysis indicates that protein expression profi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2015-12-10 | MSV000079417 | MassIVE
TranscriptomicsFacing warm temperatures during migration M-bM-^@M-^S cardiac mRNA responses of two adult sockeye salmon Oncorhynchus nerka populations to warming and swimming challenges
The main findings of the current study were that exposing adult sockeye salmon Oncorhynchus nerka to a warm temperature that they regularly encounter during their river migration induced an mRNA-level heat shock response that is exacerbated with swimming. Similar immune defense-related responses wer...
ORGANISM(S): Oncorhynchus nerka 
2013-12-15 | E-GEOD-50054 | biostudies-arrayexpress
TranscriptomicsSalmonid host response to Hematopoietic Necrosis virus: cellular receptors, viral control and novel pathways of defence
The transcriptional response of Atlantic salmon (Salmo salar) to infectious hematopoietic necrosis (IHN) virus was elucidated using 16,008 gene GRASP cDNA microarrays. S. salar were exposed to the IHN virus in a waterborne challenge, and kidney samples from five fish sampled on each of days 0, 1, ...
ORGANISM(S): Salmo salar 
2007-03-30 | E-GEOD-6924 | biostudies-arrayexpress
TranscriptomicsSalmon Spawning Migration: Metabolic Shifts and Environmental Triggers
A large-scale functional genomics study revealed shifting energy generating processes in white muscle during the final 1,300 km migration of wild sockeye salmon to their spawning grounds in the Fraser River, British Columbia. In 2006, Lower Adams stock sockeye salmon ceased feeding after passing th...
ORGANISM(S): Oncorhynchus nerka 
2010-05-15 | E-GEOD-13657 | biostudies-arrayexpress
TranscriptomicsToxicity of atrazine and nonylphenol in juvenile rainbow trout (Oncorhynchus mykiss): Effects on general health, disease susceptibility and gene expression
Atrazine (ATZ) and nonylphenol (NP) are commonly identified contaminants in aquatic habitats; however, relatively few studies have considered the impact of these endocrine disrupters on immune function. This study examined the immunotoxicological effects of ATZ and NP at multiple levels of biologic...
ORGANISM(S): Oncorhynchus mykiss 
2012-11-01 | E-GEOD-38726 | biostudies-arrayexpress
GenomicsInvestigation of the role of histone modification propagating activity of GLP
We use ChIP-Seq and RNA-Seq technology to profile the H3K9me2 modification and transcription under different conditions of GLP activity. GLP and G9a are major H3K9 dimethylases, and are essential for mouse early embryonic development. Here we report that GLP and G9a possess intrinsic histone methyla...
ORGANISM(S): Mus musculus 
2015-01-20 | E-GEOD-54412 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells (HSCs), progenitor cells (HPCs), and granulocytes somatic cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer
Since the creation of Dolly, the first sheep cloned via somatic cell nuclear transfer (SCNT), in 1997, more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1-5%) is that the clones are likely derived from adult stem...
ORGANISM(S): Mus musculus 
2008-06-14 | E-GEOD-5677 | biostudies-arrayexpress
Proteomicsgp78-regulated KAP1 phosphorylation induces radioresistance in breast cancer by facilitating PPP1CC/PPP2CA ubiquitination
To identify the target protein of gp78 that regulates KAP1 phosphorylation, we conducted an analysis using liquid chromatography–tandem mass spectrometry (LC–MS/MS), which identified several putative target proteins.
ORGANISM(S): Homo Sapiens 
2024-08-13 | PXD054846 |
TranscriptomicsSingle Cell Sequencing Identifies Key Epigenetic Regulators in Nuclear Transfer Mediated Reprogramming [RNA-seq]
Differentiated cell can be reprogrammed into totipotent embryo through somatic cell nuclear transfer (SCNT). However, this process is highly inefficient and most cloned embryos arrest at certain developmental stages. Through single cell sequencing combined with embryo biopsy, here we generate a glob...
ORGANISM(S): Mus musculus 
2016-05-17 | E-GEOD-70605 | biostudies-arrayexpress
ProteomicsAn essential maternal PADI6-UHRF1-UBE2D (MPU) complex regulates protein ubiquitination during oocyte maturation and embryogenesis
100 WT or Padi6Null MII oocytes for MS samples from three independent experiments and IP samples in 500 MII oocytes from one experiment were used for Mass spectrometry analysis.
ORGANISM(S): Mus Musculus 
2025-01-25 | PXD060230 |
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