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SPF leghorn chickens were infected with C. jejuni. The cecum were collected at 8h post infection for total RNA isolation. The significantly expressed microRNAs between infected and non-infected chickens were identified through Solexa sequencing technology. Two libraries were made from each group, in...
ORGANISM(S): Gallus gallus 
To determine the transcriptional response to Salmonella enterica serovar Enteritidis (SE) infection, a newly developed chicken 44K Agilent array was used to analyze total RNA of heterophils from SE-resistant (line A) and SE–susceptible chickens (line B), treated with in vitro infection of SE (I) o...
ORGANISM(S): Gallus gallus 
Chicken 60-mer oligonucleotide microarray, including 39854 cDNA and ESTs, entire Marek’s disease virus and avian influenza virus genomes, and 150 chicken microRNAs, was developed. Cecal tonsil, ileum, liver and spleen from 6 chickens were selected for hybridization to validate the microarray perform...
ORGANISM(S): Gallus gallus 
Briefly, each cecum was ground to powder in liquid nitrogen, dissolved in lysis buffer with 150 µL of radioimmunoprecipitation assay buffer containing 1 mM PMSF and a protease inhibitor cocktail (CST, USA), and then subjected to sonicating. The cellular debris was removed by centrifugation. The conc...
ORGANISM(S): Gallus Gallus 
2023-12-01 | PXD047433 |
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