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UnknownGenome-Wide Identification Analysis of the MAPKKK Gene Family in Cotton and Its Role in Development and Stress Response.
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S-EPMC12841829 | biostudies-literature
ProteomicsMass spectrometry analysis of USP38 interactors
After ectopic expression of Flag-USP38 or an empty vector in HEK293T cells for 48 hours, cells were lysed, and the supernatant after lysis was added to magnetic beads coupled with Flag antibodies. The mixture was incubated at 4°C overnight for pull-down. Subsequently, the magnetic beads were washed ...
ORGANISM(S): Homo Sapiens 
2024-08-26 | PXD055320 |
ProteomicsDiscovery of 180055 as a PROTAC degrader for Treating Cancers
PARP1 is an abundant nuclear protein that is involved in a number of biological processes linked to cellular stress responses. PARP1 inhibitors (PARPi) are known to kill tumor cells via two mechanisms (PARP1 catalytic inhibition and PARP1 trapping). In this work we discovered a PROTAC degrader of PA...
ORGANISM(S): Homo Sapiens 
2023-05-18 | PXD042311 |
ProteomicsMass spectrometry-based protein interactome and phosphoproteomics data upon NRDE2 overexpression
The HEK293T cells transiently expressing Flag-tagged empty vector or Flag-NRDE2-WT were lysed in NETN buffer (10 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1 mM EDTA, and 0.5% NP-40) and immunoprecipitated with anti-Flag M2 agarose (M8823; Sigma-Aldrich, MO, USA). After washing four times with the high stri...
ORGANISM(S): Homo Sapiens 
2024-03-31 | PXD051117 |
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