For the pulse-chase experiment, E. coli BW25113 and its isogenic delta prmA strains were grown in M9 minimal medium and L-Lysine-2HCl (13C6, 15N2) was added to cultures in exponential and stationary phase. The cells were cultured for an additional 2 hr and harvested. Proteins from the wild-type and ...
ORGANISM(S): Escherichia Coli Bw25113 (ncbitaxon:679895)