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TranscriptomicsExpression profiling of exposure to phorbol myristate acetate - PMA and FOXK2 depletion with siRNA
Expression profiling was done of exposure to phorbol myristate acetate -PMA and FOXK2 depletion by siRNA transfection. This was done on the human osteosarcoma U2OS cell line which stably expresses FOXK2. PMA is an inducer of AP1 activity.
ORGANISM(S): Homo sapiens 
2011-11-03 | E-MEXP-3107 | biostudies-arrayexpress
TranscriptomicsExpression profiling of FOXK2 depletion by siRNA
Expression profiling was done of FOXK2 depletion by siRNA transfection. This was done on the human osteosarcoma U2OS cell line (which stably expresses FOXK2).
ORGANISM(S): Homo sapiens 
2011-11-03 | E-MEXP-3106 | biostudies-arrayexpress
TranscriptomicsFOXK2 ChIP-chip
Chromatin immunoprecipitation of FOXK2 (tagged with Flag and His tags) in U2OS cells detected by Affymetrix human promoter array 1.0R.
ORGANISM(S): Homo sapiens 
2011-11-03 | E-MEXP-3116 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of intestine from mice infected with three isolates of Trichuris muris for 7 days to determine early changes in gene expression that may later determine the outcome of infection
Trichuris muris, the mouse whipworm is used as a laboratory model of the human parasite T. trichuira - a gastrointestinal nematode. Three laboratory isolates of T. muris exist: the E, J and S isolates. Previous data has shown that the S isolate survives to chronicity in C57BL/6 mice unlike the E and...
ORGANISM(S): Mus musculus 
2010-02-26 | E-MEXP-1795 | biostudies-arrayexpress
no omics definedTranscription profiling of human Ecr293 cells transfected with ponasterone A to identify ETS-domain transcription factor Elk-1 target genes
ETS-domain transcription factor Elk-1 target genes identified using an inducible dominant-negative expression system. Here, we use a stably-transfected EcR293 cell line containing a ponasterone A
ORGANISM(S): Homo sapiens 
2009-02-26 | E-MEXP-1526 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of bovine nucleus pulposus, carticular cartilage and annulus fibrosus cells to further determine expression in normal and degenerate human intervertebral disc
The aim of this transcription profiling study was to identify novel genes that could be used to distinguish bovine Nucleus pulposus (NP) cells from articular cartilage (AC) and annulus fibrosus (AF) cells and to further determine their expression in normal and degenerate human intervertebral disc (I...
ORGANISM(S): Bos taurus 
2010-03-05 | E-MEXP-2291 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of human Hela cells grown under serum starved condtions to identify new targets genes for ETS-domain transcription factor Elk-1
Chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip) was carried out to identify new target genes for ETS-domain transcription factor Elk-1. Experiment was done in Hela cells under serum starved conditions.
ORGANISM(S): Homo sapiens 
2009-08-11 | E-MEXP-1527 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of human HeLa cells grown under serum starved conditions to identify new target genes for ETS-domain transcription factor Elk-1
Chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip) was carried out to identify new target genes for ETS-domain transcription factor Elk-1. Experiment was done in Hela cells under serum starved conditions.
ORGANISM(S): Homo sapiens 
2009-08-11 | E-MEXP-2084 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of homozygous Commd1 null mouse embryos to elucidate the underlying mechanism leading to embryonic lethality
A gene expression study using microarray analysis was performed to elucidate the underlying mechanism leading to embryonic lethality in homozygous Commd1 null (Commd1-/-) mouse embryos. A gene expression profile of 9.5 dpc Commd1-/- embryos were generated and were compared to a gene expression profi...
ORGANISM(S): Mus musculus 
2006-09-01 | E-MEXP-832 | biostudies-arrayexpress
TranscriptomicsMutation of ZMYM2 SIMs
The interaction between SUMO and ZMYM2 is mediated through specific motifs found in ZMYM2 that are called SIMs (SUMO interacting motifs). We found/characterised three SIMs in ZMYM2 and in vitro experiments showed that mutation in these residues prevent the interaction with SUMO. Microarray transcrip...
ORGANISM(S): Homo sapiens 
2015-08-06 | E-MEXP-3880 | biostudies-arrayexpress
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