Cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification of C. sinensis leaves. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CW...
In this project, we used biotin-labeled compound 1 to pull down proteins in U2OS cell lysates with or without the competition of free compound 1, and then to identify the cellular binding of compound 1.
Based on the gene ontology (GO) results, we found that proteins associated with responses or processes related to stimulus, immune system, signalling, cell killing, and antioxidant activity were more up-regulated after MiBaMc treatment. In contrast, proteins that related to cell integrity and cell g...