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Low molecular weight RNA from conventional T cells and T reg cells was hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell type comparison Six arrays representing three biological replicates and dyeswaps of each.
ORGANISM(S): Mus musculus 
Activated T cells were transduced with either Foxp3-IRES-GFP or IRES-GFP control vector. Low molecular weight RNA from cells was extracted 72h or 96h later and hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell type comparison Four arra...
ORGANISM(S): Mus musculus 
T cells were activated for 1, 3 or 10 days using plate-bound anti-TCR and anti-CD28. Low molecular weight RNA from conventional T cells and activated T cells was hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell state analysis Five arr...
ORGANISM(S): Mus musculus 
A study of the miRNA profile CD4 T cells compared to T reg cells; of activated and naive T cells; and the miRNA profile conferred by enforced expression of Foxp3. Keywords: cell type comparison Fifteen arrays broken down into three broader experiments as referenced by series GSE6003 - a comparison o...
ORGANISM(S): Mus musculus 
Development of a method for separation and parallel sequencing of the genomes and transcriptomes of single cells.
The ability to simultaneously sequence the genome and transcriptome of the same single cell offers a powerful means to dissect functional genetic heterogeneity at the cellular level. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. ...
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