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ProteomicsA genome-wide CRISPR interference screen using an engineered trafficking biosensor reveals a role for DNAJC13 in opioid receptor downregulation
G protein-coupled receptor (GPCR) activity is critically regulated by trafficking through the endo-lysosomal pathway. Identifying the genes involved in GPCR trafficking is challenging due the complexity of sorting operations and low affinity protein-receptor interactions. Here we show that the engin...
ORGANISM(S): Homo sapiens (Human) 
2024-09-03 | PXD044921 | Pride
Endosomal sorting Isobaric labeling Apex2 Proximity labeling Gpcr Endolysosome Quantitative proteomics Dnajc13 Opioid receptor 
ProteomicsAn Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells - B2AR_DOR
SRM assays were generated for selected interactors of B2AR and DOR(MSV000084857), as well as for localization controls and ribosomal proteins (RPL18A, RPL28, RPL3, RPL35A, RPL6) as internal controls for normalization (Table S5 in publication). SRM assay generation was performed using Skyline. For al...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-02-17 | MSV000084966 | MassIVE
ProteomicsAn Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells - B2AR_localization
SRM assays were generated for selected interactors of B2AR and DOR (MSV000084857), as well as for localization controls and ribosomal proteins (RPL18A, RPL28, RPL3, RPL35A, RPL6) as internal controls for normalization (Table S5 in publication). We selected localization markers for different cellular...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-02-17 | MSV000084967 | MassIVE
ProteomicsA method for spatially and temporally resolved protein network interrogation in living cells
Cells operate through protein interaction networks organized in space and in time, but current methods to interrogate such biology typically resolve only one of these dimensions. Here, we describe a method to resolve both dimensions simultaneously using proximity labeling mediated by engineered asco...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-01-29 | MSV000084857 | MassIVE
ProteomicsProfiling the diversity of agonist-selective effects on the proximal proteome environment of G protein-coupled receptors
G-protein coupled receptors (GPCRs) regulate various aspects of cellular behavior and represent actionable targets for drug discovery. Activation of specific signaling pathways downstream of G-protein coupled receptors (GPCRs) or targeting the receptors at selected cellular locations has the potenti...
ORGANISM(S): Homo Sapiens 
2024-03-31 | PXD031393 | panorama
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