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Analysis of mRNA stability in S. cerevisiae in connection to hyperosmotic stress at different time points following mild hyperosmotic shock in Saccharomyces cerevisiae cells.
ORGANISM(S): Saccharomyces cerevisiae 
Strain GS115 transformed with the empty vector pGAPHIS (a histidine prototrophic isogenic strain of GS115) was compared to Strain GS115 HAC1, constitutively overproducing the activated form of S. cerevisiae Hac1
ORGANISM(S): Pichia pastoris 
The replication of eukaryotic chromosomes is organized temporally and spatially within the nucleus through epigenetic regulation of replication origin function. The characteristic initiation timing of specific origins is thought to reflect their chromatin environment or sub-nuclear positioning, how...
ORGANISM(S): Saccharomyces cerevisiae 
We compared the performance of conventional RNAseq with RNA Capture Sequencing (CaptureSeq) to assemble and quantify known RNA spike-Ins and human transcripts. We find CaptureSeq to be superior for the detection and quantification of the 37% lowest expressed genes, and comparable for the next 45% of...
ORGANISM(S): Homo sapiens 

INTRODUCTION: Premature adrenarche (PA) for long time was considered a benign condition but later has been connected to various diseases in childhood and adulthood which remains controversial.

OBJECTIVE: To investigate the effect of premature adrenarche ...

2022-10-18 | MTBLS2387 | MetaboLights
Experiment description To identify FoxM1-regulated genes, we carried out gene expression profile analysis after inducible activation of a FoxM1-ER fusion protein using high-density human cDNA microarrays. We constructed a stable line of U2OS cells expressing full-length HA-FoxM1 fused at its C-term...
ORGANISM(S): Homo sapiens 
Age-related and/or high caloric intake driven changes in visceral adipose tissue contribute to the development of diabetes and cardiovascular disease. The lack of reliable, high-throughput methods to analyze the adipose tissue protein composition has limited our understanding of the protein network...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-07-03 | MSV000082569 | MassIVE
We used RNAseq to identify how membrane fatty acid changes might impact and explain the virulence defect during host infection. WT and mFabT expression was compared in THY, and in THY-Tween (as C18:1Δ9 source), which to activates WT FabT repression. RNA isolation and Illumina RNA-seq sequencing: GA...
ORGANISM(S): Streptococcus pyogenes 

Anthocyanins are economically valuable phytochemicals of significant relevance to human health. Industrially extracted from multiple fruit and vegetable sources, anthocyanin yield and profiles can vary between sources and growing conditions. In this study, we focused on three purple-fleshed and o...

2021-09-01 | MTBLS2956 | MetaboLights
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