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ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive proteome: temperature range thermal profiling experiment
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033713 | Pride
Toxoplasma Parasite Calcium signaling Cetsa Tpp Apicomplexan Systems biology Thermal profiling 
ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive proteome: concentration range thermal profiling experiment 2
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033650 | Pride
Toxoplasma Thermal proteome profiling Proteomics Parasite Calcium signaling Cetsa Tpp Apicomplexan Systems biology 
ProteomicsImmunoprecipitation of CLAMP-HA and CLAMPΔPRD-HA from the parasite T. gondii
Apicomplexan parasites discharge specialized organelles called rhoptries upon host cell contact to mediate invasion. The events that drive rhoptry discharge are poorly understood, yet essential to sustain the apicomplexan parasitic life cycle. Rhoptry discharge appears to depend on proteins secreted...
ORGANISM(S): Toxoplasma gondii GT1 
2023-10-30 | PXD044091 | Pride
Rhoptry secretion. Toxoplasma gondii Clamp 
ProteomicsThermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1)
Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extra...
ORGANISM(S): Toxoplasma gondii GT1 
2020-07-13 | PXD018810 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa 
ProteomicsGlycan bead precipitation of proteins from Toxoplasma gondii lysate
Apicomplexan parasites use two sets of specialized organelles to invade mammalian host cells, the micronemes and the rhoptries. Microneme proteins mediate adhesion and interaction with host cell surfaces and upon appropriate contact mediate discharge of rhoptry organelles. Injection of rhoptry conte...
ORGANISM(S): Toxoplasma gondii RH 
2026-08-18 | PXD079900 | Pride
Toxoplasma gondii Galactose Mannose Invasion Lactose Microneme 
ProteomicsT. gondii TIR1 strain temperature range thermal profiling experiment
Toxoplasma gondii is a single-celled eukaryotic parasite that chronically infects a quarter of the global population. In recent years, phenotypic screens have identified compounds that block parasite replication. Unraveling the complexity of pathogenesis and molecular mechanisms and pathways perturb...
ORGANISM(S): Toxoplasma gondii RH 
2023-03-11 | PXD024912 | Pride
Parasite Tpp Cetsa Toxoplasma 
ProteomicsThermal proteome profiling of Toxoplasma gondii treated with enhancer 1 (ENH1)
reviously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extrac...
ORGANISM(S): Toxoplasma gondii GT1 
2023-08-21 | PXD018818 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa 
ProteomicsIdentification of proteins exhibiting TgDegP2-responsive thermal stability
To broadly explore artemisinin susceptibility in apicomplexan parasites, we used genome-scale CRISPR screens recently developed for Toxoplasma gondii to discover sensitizing and desensitizing mutations. From these screens, we identified the mitochondrial protease DegP2, which appeared gDegP2-respons...
ORGANISM(S): Toxoplasma gondii GT1 
2020-08-28 | PXD019917 | Pride
Thermal proteome profiling Toxoplasma gondii Cetsa Artemisinin susceptibility 
ProteomicsDe novo mapping of the apicomplexan Ca2+-responsive phosphoproteome
Apicomplexan parasites cause persistent mortality and morbidity worldwide through diseases including malaria, toxoplasmosis, and cryptosporidiosis. Ca2+ signaling pathways have been repurposed in these eukaryotic pathogens to regulate parasite-specific cellular processes governing the transition bet...
ORGANISM(S): Toxoplasma gondii RH 
2022-10-15 | PXD033765 | Pride
Toxoplasma Parasite Calcium signaling Phosphoproteomics Apicomplexan Systems biology 
ProteomicsTwo distinct SWI/SNF complexes direct chromatin-linked transcriptional programs in Toxoplasma
Chromatin remodeling complexes dynamically modify DNA accessibility to mediate changes in gene expression during eukaryotic cell cycle progression, developmental transitions, and environmental adaptation. Higher eukaryotes have multiple remodeler subtypes based on the incorporation of different ATPa...
ORGANISM(S): Toxoplasma gondii GT1 
2026-04-26 | PXD066820 | Pride
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