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TranscriptomicsTranscriptome profile of subcutaneous adipocytes isolated from obese vs. lean postmenopausal women
Adipocytes isolated from lean and obese postmenopausal women with no significant differences in metabolic syndrome parameters demonstrate changes in multiple inflammatory, metabolic and structural gene families. Purified adipocyte samples were isolated from subcutaneous adipose tissue surgical biops...
ORGANISM(S): Homo sapiens 
2013-02-02 | E-GEOD-44000 | biostudies-arrayexpress
GenomicsDerivation of pre-X inactivation human embryonic stem cells under physiological oxygen concentrations
The presence of two active X chromosomes (XaXa) is a hallmark of the ground state of pluripotency specific to murine embryonic stem cells (ESCs). Human ESCs invariably exhibit signs of X chromosome inactivation (XCI) and are considered developmentally more advanced than their murine counterparts. W...
ORGANISM(S): Homo sapiens 
2010-05-28 | E-GEOD-21141 | biostudies-arrayexpress
ProteomicsMS quantification of circulating CFP-10, p24 and VP40
Liquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising met...
ORGANISM(S): Ebola Virus Mycobacterium Tuberculosis Sudan Ebolavirus Human Immunodeficiency Virus 1 
2021-11-09 | PXD021149 | panorama
ProteomicsMRM quantification of circulating CFP-10 in TB patients
Liquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising met...
ORGANISM(S): Mycobacterium Tuberculosis 
PXD021150 | panorama
ProteomicsSensitive detection of HIV-1 and Mycobacterium tuberculosis peptides in blood for rapid diagnosis of co-infected individuals by immuno-affinity liquid chromatography tandem mass spectrometry
We describe a blood-based immuno-affinity liquid chromatography tandem mass spectrometry (ILM) assay that quantifies proteotypic peptides derived from the Mtb virulence factor CFP10 and the HIV-1 capsid protein p24 to simultaneously diagnose HIV-1 infection and TB disease and to monitor their real-t...
ORGANISM(S): Homo Sapiens 
2023-11-15 | PXD043191 | panorama
ProteomicsBlood-based Diagnostics of Pediatric Tuberculosis: A Prospective Case-controlled Study
Background Pediatric tuberculosis (TB) diagnosis is often challenging due to difficultly obtaining diagnostic respiratory specimens, which may contain low concentrations of Mycobacterium tuberculosis (Mtb) bacilli. However, we have reported that an immuno-affinity liquid chromatography-tandem mass s...
ORGANISM(S): Homo Sapiens 
PXD053274 | panorama
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