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Biotechnology has transformed the production of various chemicals and pharmaceuticals due to its efficient and selective processes, but it is inherently limited by its use of live cells as 'biocatalysts.' Cell-free expression (CFE) systems, which use a protein lysate isolated from whole cells, have ...
2020-06-02 | MTBLS1079 | MetaboLights
The aim of this study was to provide proof of principle as to whether probiotic bacteria or their extracts, could stimulate cutaneous wound healing. To this end, we have used a keratinocyte monolayer scratch assay which assesses one important aspect of wound healing, re-epithelialization. Primary hu...
ORGANISM(S): Homo sapiens 
Pneumonia is a serious problem worldwide. We recently demonstrated that innate defense mechanisms of the lung are highly inducible against pneumococcal pneumonia. To determine the breadth of protection conferred by stimulation of lung mucosal innate immunity, and to identify cells and signaling pa...
ORGANISM(S): Mus musculus 
Gene Expression changes in BMDCs stimulated with H. pylori vs. E. coli. The hypothesis tested was that the gene expression profile in BMDCs stimulated with H. pylori lysate will be less inflammatory than BMDCs stimulated with E. coli. BMDCs were generated from bone marrow of wild type female C57BL/6...
ORGANISM(S): Mus musculus 
The field of metabolic engineering has yielded remarkable accomplishments in using cells to produce valuable molecules, and cell-free expression (CFE) systems have the potential to push the field even further. However, CFE systems still face some outstanding challenges, including endogenous metaboli...
2023-02-22 | MTBLS2630 | MetaboLights
Protein production using processed cell lysates, defined precursor cocktails and target specific additives has become a core technology in the field of synthetic biology. Due to their open nature, these artificial systems are excellent to produce difficult proteins such as toxins or membrane protein...
ORGANISM(S): Escherichia coli 
2017-09-29 | PXD004646 | Pride
Coccolithophore viral lysate Raw sequence reads
Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. Fo...
ORGANISM(S): Homo sapiens 
Quality evaluation of Enzymatic Methyl-seq library constructed using crude cell lysate
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